High Performance Liquid Chromatography

Description

High performance liquid chromatography (HPLC) has long been recognized as one of the most useful and versatile analytical techniques. It has now progressed from being a highly expensive method of analysis to a routine technique with wide applications. Consequently there is a requirement in many chemistry and chemistry-related courses for students to acquire a detailed understanding of the principles and practice of HPLC.

Written in a manner suitable for undergraduate students studying analytical chemistry and learning about chromatographic analytical techniques applied to pharmaceutical analysis, biochemistry and related disciplines, High-performance Liquid Chromatography: Fundamental Principles and Practice introduces the fundamentals of HPLC. Loosely structured in three parts, the text begins with a thorough introduction of the subject and then progresses through the essential knowledge of the instrumentation needed for HPLC. The final part covers with the applications of HPLC in real-world situations. Developed by a team of international experts from a wide cross-section of disciplines, the text is relevant to a wide range of courses.

Contents

INTRODUCTION, W.J. Lough and I.W. Wainer

Analysis and Chromatography

Historical Development of Chromatography

HPLC Today

EFFICIENCY, RETENTION, SELECTIVITY, AND RESOLUTION IN CHROMATOGRAPHY, C.M. Riley

Introduction

Chormatographic Mobility

Peak Shape

Retention Relationships

Band Broadening and Column Efficiency

Separation

MODES OF CHROMATOGRAPHY, C.M. Riley

Terminology

Chromatographic Format

Models of Retention

Choice of Mode of Chromatography

Normal-Phase Liquid Chromatography

Reversed-Phased Liquid Chromatography

Ion-Exchange Chromatography

Size-Exclusion Chromatography

SUPPORT MATERIALS AND SOLVENTS, P. Hambleton

Adsorption Chromatography

Chemically Bonded Silica

Polymer Packings

Porous Graphitic Carbon

Solvents for HPLC

INSTRUMENTATION: PUMPS, INJECTORS, AND COLUMN DESIGN, T. Noctor

Introduction

Types of HPLC Pump

Approaches to the Reduction of Flow Pulsation

The Modern HPLC Pump

Valve Injectors

Column Dimemsions

Column Inlet/Outlet

Connecting Tubing and Unions

Conclusion

INSTRUMENTATION: DETECTORS AND INTEGRATORS, D.K. Lloyd

Ideal Detectors and Real Detectors

Detector Performance Criteria

The UV-Visible Absorbance Detector

The Fluorescence Detector

The Refractive Index Detector

Electrochemical Detectors

Other Detection Methods

Integrators

METHOD DEVELOPMENT AND QUANTITATION, W.J. Lough and I.W. Wainer

Method Development

Optimisation

Relative Assays

Method Validation

Types of Quantitative Method

SAMPLE PREPARATION, D. Stevenson

Introduction

Sample Preparation Procedures

Matrix Properties

Analyte Propeties

Examples of Applications

Automation

Concluding Remarks

POLYMER ANALYSIS, A.J. Handley

Introduction

Chromatography Modes Used for Polymer Analysis

Size Exclusion Chromatography

Other Modes of Chromatography

HPLC IN BIOMEDICAL AND FORENSIC ANALYSIS, D. Perrett and P. White

Introduction

Biomedical Analysis

Forensic Analysis

ENVIRONMENTAL ANALYSIS, P.J. Renne

Introduction

HPLC Mode

Sample Preparation

Choice of Column

Pumping System

Detectors

Sample Introduction

Analytes

Miscellaneous

Suggested Protocol

Conclusions

FOOD, ORGANIC, AND PHARMACEUTICAL APPLICATIONS, W.J. Lough and I.W. Wainer

Introduction

Bulk Substances

Products or 'Formulations'

Conclusion

Related Subjects

1. Separation Science/Chromatography
2. Industrial & Applied Chemistry
3. Chromatography
4. Industrial Chemistry