Biomolecular EPR Spectroscopy
By Wilfred Raymond Hagen
Published December 22nd 2008 by CRC Press – 248 pages
Published December 22nd 2008 by CRC Press – 248 pages
Comprehensive, Up-to-Date Coverage of Spectroscopy Theory and its Applications to Biological Systems
Although a multitude of books have been published about spectroscopy, most of them only occasionally refer to biological systems and the specific problems of biomolecular EPR (bioEPR). Biomolecular EPR Spectroscopy provides a practical introduction to bioEPR and demonstrates how this remarkable tool allows researchers to delve into the structural, functional, and analytical analysis of paramagnetic molecules found in the biochemistry of all species on the planet.
A Must-Have Reference in an Intrinsically Multidisciplinary Field
This authoritative reference seamlessly covers all important bioEPR applications, including low-spin and high-spin metalloproteins, spin traps and spin lables, interaction between active sites, and redox systems. It is loaded with practical tricks as well as do’s and don’ts that are based on the author’s 30 years of experience in the field. The book also comes with an unprecedented set of supporting software designed with simple graphical user interfaces that allow readers to tackle problems they will likely encounter when engaged in spectral analysis.
Breaking with convention, the book broaches quantum mechanics from the perspective of biological relevance, emphasizing low-symmetry systems. This is a necessary approach since paramagnets in biomolecules typically have no symmetry. Where key topics related to quantum mechanics are addressed, the book offers a rigorous treatment in a style that is quick-to-grasp for the non expert. Biomolecular EPR Spectroscopy is a practical, all-inclusive reference sure to become the industry standard.
"EPR is being increasingly used to investigate biological systems, but there is currently no text that biologists, biochemists, and bioinorganic chemists can use to understand the experimental and theoretical aspects of this technique without being experts in quantum mechanics… provides a valuable introductory text that can be recommended… The author is a prolific writer with a clear and concise writing style."
—Michael K. Johnson, Department of Chemistry, University of Georgia, Athens, USA
BASICS
Introduction
The Spectrometer
The Sample
Experimental Key Parameters
Resonance Condition
Analysis
THEORY
Energy Matrices
Biological Spin Hamiltonians
Conformational Distributions
SPECIFIC EXPERIMENTS
Aqueous Solutions
Interactions
High Spins Revisited
Black Box Experiments
Strategic Considerations
References
Index
Name: Biomolecular EPR Spectroscopy (Hardback) – CRC Press
Description: By Wilfred Raymond Hagen.
Comprehensive, Up-to-Date Coverage of Spectroscopy Theory and its Applications to Biological Systems
Although a multitude of books have been published about spectroscopy, most of them only occasionally refer to biological systems and the specific...
Categories: Chemical Spectroscopy, Biochemistry, Bioscience