A Laboratory Course in Tissue Engineering

Getting Started in the Lab

Lab Safety

Essential Lab Skills for Tissue Engineers

Isolation of Primary Chondrocytes from Bovine Articular Cartilage

Measuring and Modeling Growth of a Cell Population

Purification of a Cell Population Using Magnetic Cell Sorting

Decellularized Matrices for Tissue Engineering

Effect of Plating Density on Cell Adhesion to Varied Culture Matrices

Dynamic versus Static Seeding of Cells onto Biomaterial Scaffolds

Cell Patterning Using Microcontact Printing

Measuring and Modeling the Motility of a Cell Population Using an Under-Agarose Assay

Characterizing Matrix Remodeling through Collagen Gel Contraction

Effect of Substrate Stiffness on Cell Differentiation

Effect of Culture Configuration (Two versus Three Dimensions) on Matrix Accumulation

Combining In Silico and In Vitro Techniques to Engineer Pluripotent Stem Cell Fate

The FahraeusLindqvist Effect: Using Microchannels to Observe Small Vessel Hemodynamics

Examining Single-Cell Mechanics Using a Microfluidic Micropipette Aspiration System

Contribution of Tissue Composition to Bone Material Properties

Technical Communication: Presenting Your Findings

Appendix 1: Trypsinizing a Cell Monolayer

Appendix 2: Counting Cells with a Hemacytometer

Appendix 3: PicoGreen^® DNA Assay

Appendix 4: Dimethylmethylene Blue (DMMB) Assay for Sulfated Glycosaminoglycans

Appendix 5: Microfluidic Device Design and Fabrication

Index

Biography

Melissa Kurtis Micou , Ph.D., is a lecturer in the Department of Bioengineering at the University of California, San Diego. She has taught tissue-engineering lecture and lab courses for undergraduate students for the past ten years. Dawn M. Kilkenny , Ph.D., is an assistant professor at the Institute of Biomaterials and Biomedical Engineering (IBBME), University of Toronto, and is academic advisor to the IBBME undergraduate teaching laboratory. Her research interests include cellular signaling, fluorescent protein technology, and microscopy.

"The book is well organized to teach cell culture and tissue engineering experiments to novice and experienced students. There is a quantitative emphasis in the book that strengthens the ‘engineering’ part of tissue engineering."
—Ann Saterbak, Rice University, Houston, Texas, USA

"… an excellent handbook for graduate students and investigators who are new in tissue engineering area. … The approaches and topics selected are appropriate for not only undergraduate but also graduate students and new investigators in this area."
—Sha Jin, University of Arkansas, Fayetteville, USA

"This book has a protocol-like style and can actually be used directly by teachers to prepare lab courses as well as by people with lab experience that enter the field of tissue engineering. … provides students with good insight in methods, techniques and approaches in the field of tissue engineering."
—Gerjo J.V.M. van Osch, Erasmus MC, University Medical Center Rotterdam, the Netherlands

"… a much-needed book for undergraduate bioengineering curricula. Tissue engineering is a topic best learned through practice, and this book just might take the fear out of offering a laboratory course on the subject."
—Michael J. Moore, Ph.D., Tulane University, New Orleans

"… provides comprehensive coverage of laboratory techniques in tissue engineering, including detailed experimental protocols."
—Adam Higgins, Oregon State University, Corvallis