This book identifies the converging fundamental knowledge bases underpinning the field of 3D cell culture and moves it from art to science. It provides a meaning of three-dimensionality and how it can be assayed. It also provides the value proposition for 3D cell culture in the major application areas. For example, 3D cell cultures are increasingly being used in therapeutic drug discovery programs to screen compounds with promise. The author uses carefully chosen applications to complement incorporated principles.
Table of Contents
Introduction. Complex Physiological Relevance. Cells of Muscle Tissue Origin. Cells of Epithermal Tissue Origin. Cells of Nerve Tissue Origin. Cells of Connective Tissue Origin. Microenvironmental Factors. Chemical/Biochemical Microenvironmental Factors. Size/Space Microenvironmental Factors. Physical Property/Applied Force Microenvironmental Factors. Three-Dimensionality Biomarkers. The Case for Cytokines. Discovery and Validation. Applications. Enabling technologies. Research Model Examples. Drug Discovery Examples. Therapeutic Examples. Three-Dimensionality Assay.
William S. Kisaalita, PhD, is a professor and Biological Engineering Program Coordinator in the College of Engineering, University of Georgia, where he also directs the Cellular Bioengineering Laboratory.