1st Edition

Advances in Animal Disease Diagnosis

Edited By Suresh Kumar Gahlawat, Sushila Maan Copyright 2021
    336 Pages 32 Color Illustrations
    by CRC Press

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    Advances in Animal Disease Diagnosis: Infectious animal diseases caused by pathogenic microorganisms such as bacteria, fungi, and viruses threaten the health and well-being of wildlife, livestock and human populations, limit productivity and significantly increase economic losses to each sector. Pathogen de-tection is an important step for the diagnosis and successful treatment of animal diseases as well as control management in farm and field conditions. The conventional techniques employed to diagnose pathogens in livestock species are time-consuming and sometimes give inconclusive results. On the contrary, molecular techniques have the potential to diag-nose known pathogens/conditions quickly, reliably, and unequivocally as well as for novel pathogen detection. New advances in diagnostics and vaccine design using genomics have developed powerful new methods that have also set the stage for the enhanced diagnosis, surveillance, and control of infectious diseases. High-throughput sequencing (HTS), for ex-ample, uses the latest DNA sequencing platforms in the detection, identification, and detailed analysis of both pathogen and host genomes.

    This book will explore some key opportunities in the context of animal health, such as the detection of new microorganisms and the development of improved diagnosis of emerging or re-emerging diseases and other clinical conditions, viz. biosensors, nanotools, and omics technologies.


    • Details comprehensive knowledge on the latest molecular techniques for animal disease diagnosis and management
    • Examines how DNA-based diagnostic techniques will assist international efforts to control the introduction of exotic diseases into new geographic areas
    • Describes the latest molecular assays for the rapid and accurate detection of pathogens
    • Helps in working towards meeting the global challenge for sustainable food production and the eradication of poverty
    • With new biotechnological developments, this fully updated book is a treasure trove of the latest information in animal and  medical science


    Chapter 1

    Biosensor: An Advanced System for Infectious Disease Diagnosis

    Niti Chawla and Parvati Sharma

    Chapter 2

    Viral Pseudotyping: A novel tool to study emerging and transboundary viruses

    Aravindh Babu R. Parthiban, Karthik Kumaragurubaran and Dhinakar Raj Gopal


    Chapter 3

    Advanced Sensors for Animal Disease Diagnosis

    Swati Dahiya, Jyotsana Mehta and BhuvanNagpal


    Chapter 4

    Applications of metagenomics and viral genomics to investigating diseases of livestock

    Barbara Brito Rodriguez

    Chapter 5

    Toll- like Receptor of Livestock Species

    P. K. Dubey, S. Dubey, S.K. Gahlawat and R.S Kataria

    Chapter 6

    COVID-19: An Emerging Pandemic To Mankind

    Aman Kumar, Kanisht Batra, Anubha Sharma and Sushila Maan

    Chapter 7

    Application of Proteomics and Metabolomics in Disease Diagnosis

    Lukumoni Buragohain, Mayukh Ghosh, Rajesh Kumar, Swati Dahiya, Yashpal S. Malik and Minakshi Prasad

    Chapter 8

    Imaging Techniques in Veterinary Disease diagnosis

    Minakshi Prasad, Mayukh Ghosh, Suman, Harshad Sudhir Patki, Sandeep Kumar, Basanti Brar, Neelesh Sindhu, Parveen Goel, Samander Kaushik, Hari Mohan, Shafiq Syed and Rajesh Kumar

    Chapter 9

    Listeriosis in Animals: Prevalence and Detection

    Tejinder Kaur and Praveen P. Balgir

    Chapter 10

    Pyroptosis Prevalence in Animal Diseases and Diagnosis

    Praveen P. Balgir and Suman Rani

    Chapter 11

    Current Diagnostic Techniques For Influenza

    Aruna Punia, Pooja Choudhary, Sweety Dahiya, Namita Sharma, Meenakshi Balhara, Mehak Dangi and Anil Kumar Chhillar

    Chapter 12

    Diagnostic Tools For the Identification of Foot and Mouth Disease Virus

    Sweety Dahiya, Aruna Punia, Pooja Choudhary, Namita Sharma, Meenakshi Balhara, Mehak Dangi  and Anil Kumar Chhillar

    Chapter 13

    Synthetic Biology-based Diagnostics For Infectious Animal Diseases

    Naveen Kumar, Sandeep Bhatia and Yashpal Singh Malik

    Chapter 14

    Recent Trends in Diagnosis of Campylobacter Infection

    Pooja Choudhary, Aruna Punia, Sweety Dahiya, Namita Sharma, Meenakshi Balhara, Mehak Dangi and Anil Kumar Chhillar


    Chapter 15

    Recent Trends in Bovine Tuberculosis Detection and Control Methods



    Chapter 16

    Livestock Enteric Viruses: Latest Diagnostic Techniques For Their Easy and Rapid Identification

    Sushila Maan, Renu Singh, Monika Punia and Kanisht Batra


    Chapter 17

    Coronaviruses: Recent Trends and Approaches in Diagnosis and Management

    Samander Kaushik, Yashika Sharma, Sulochana Kaushik, Jaya Parkash Yadav and Minakshi Prasad


    Chapter 18

    Recombinase Polymerase Amplification (RPA): A New Approach for Disease Diagnosis

    Monika Punia, Nidhi Saini, Suresh Kumar Gahlawat and Sushila Maan


    Chapter 19

    Global Rules, Regulations and Intellectual Property Rights on diagnostic methods

    Anil Ghanghas, Sarvar Gahlawat, Neer Gahlawat and Asha Poonia



    Suresh Kumar Gahlawat, Ph.D. is presently working as Professor, Department of Biotechnology Chaudhary Devi Lal University (CDLU), Sirsa, India. He also worked in various capacities such as Dean, Research, Dean, Faculty of Life Sciences, Dean of Colleges, Dean Student’s Welfare and Chairperson, Department of Biotechnology in the same university. He received postdoctoral BOYSCAST fellowship and DBT Overseas Associateship from the Ministry of Science & Technology, Government of India for carrying out research at FRS Marine Laboratory, Aberdeen, UK. His research interests include the development of molecular diagnostic methods for bacterial and viral diseases. He published more than 70 research papers in journals of national and international repute, authored more than 06 books and supervised M.Phil and Ph.D research work of 14 students. He is active member of various international scientific organizations and societies including Association Microbiologist of India.

    Sushila Maan, Ph.D. Professor & Head at Department of Animal Biotechnology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, India. She did her Ph.D. from Royal Veterinary College, University of London, UK. She had been Post-Doctoral Fellow within the Arbovirus Molecular Research Group at the Institute for Animal Health (IAH), Pirbright, UK from 2006 – 2011. Dr Sushila Maan has made vital contributions to the extraordinary high impact success of bluetongue virus (BTV) and other pathogens’ research and surveillance including the development of innovative next generation sequencing techniques, identification of seven new Orbivirus species and the 26th serotype of BTV. Dr Maan has developed and commercialized advanced molecular diagnostic systems for detection of pathogens of livestock and wildlife importance, during twenty-four years of her research career since 1995, she has published 122 peer reviewed research articles, out of which 76 are in various international journals of repute and 46 in national journals. She has also presented her research findings at several international conferences in the U.K., France, Portugal, Italy, Holland, Australia, South Africa, China and USA. She is on scientific panel (as a referee) of various International and National journals viz. Virus Research, Plos One, Virology, Vaccine, Journal of Virology, Transboundary and emerging diseases, Indian Journal of Virology etc.

    "In this multi-authored text, contributing experts offer a broad overview of the most modern technologies—primarily molecular in scale but including an array of innovative microscopic and scanning techniques—for the detection and identification of infectious-disease organisms. In considering these novel diagnostic methods, authors generally emphasize overall utility and performance characteristics, notably sensitivity/specificity, accuracy/precision, rapidity, affordability, and ease of use. They also consider the direction of future efforts with respect to selected animal diseases. As shown in these pages, widespread application of modern technology has greatly changed the nature of animal disease diagnosis. This volume will be of considerable value to virologists, bacteriologists, molecular biologists, and others working in diagnostic facilities."

    D. A. Brass, independent scholar, Choice December 2022