Reports up-to-date research developments on purifying and isolation large organic molecules. The text provides information on high-performance liquid chromatography and capillary electrophoresis (CE) as tools for analyzing biomacromolecules and developing new biochemical and medicinal compounds. It applies biochemical separation technology to the study of macromolecules such as proteins, polysaccharides, nucleic acids and more.
Table of Contents
Analysis of HbA and some Hb variants by HPLC; analysis of posttranslational modifications in recombinant proteins by HPLC and mass spectrometry; analytical and preparative separations of peptides by capillary and free-flow zone electrophoresis; bioaffinity chromatography; characterization and partial purification of steroidongenic factors from thymic epithelial cell-conditioned medium; determination of affinity constants of lectins for sugars by affinity capillary electrophoresis; displacement chromatography of biomolecules; high performance membrane chromatography of proteins; HPLC purification of recombiant proteins; HPLC purification of recombiant proteins; isolation, purification, and characterization of human seminal plasma proteins and their immunological behaviour in vitro; isolation, purification, and structural study of allergenic proteins; multiangle light scattering combined with HPLC with examples for biopolymers; purification and characterization of connective tissue growth factor using heparin affinity chromatography; slalom chromatography - a new hydrodynamic-based chromatographic mode applicable to size-dependent separation and physiochemical analysis of large DNA molecules; simultaneous chromatographic separation of ceruloplasmin and serum amine oxidase; fast, single-step affinity chromatography purification of haemoglobin.