Chapman and Hall/CRC
The activity of many biopharmaceutical polymers is dependent on conformation, and the next several years will see increased interest in the conformational analysis of these polymers resulting from the development of biosimilar or "follow-on" biological products. While a wide variety of approaches to analysis exists, finding the most viable ones wou
Introduction to Biopharmaceutical Conformational Analysis: Issues and Methods. Comparability of Biotechnological/Biological Products and Biological Generics. Application of Native Electrophoresis for the Study of Protein Conformation. Affinity Chromatography Including Hydrophobic Interaction Chromatography in the Study of Biopolymer Conformation. Size-Exclusion Chromatography and Biomolecular Conformation. Use of Analytical Ultracentrifugation to Study Biomolecular Conformation. Use of Differential Scanning Calorimetry to Measure Conformational Change in Proteins and Other Biomacromolecules. Light Scattering and Biomacromolecular Conformation. Use of Luminescence to Measure Conformational Change in Biopharmaceuticals with Emphasis on Protein and Protein Drug Products. Near-Infrared Spectroscopy and Macromolecular Conformation. The Use of Mid-Infrared and Fourier Transform Infrared Spectroscopy to Study Conformation of Biomacromolecules. Use of Raman Spectroscopy to Evaluate Biopharmaceutical Conformation. Use of UV-VIS Spectrophotometry for the Characterization of Biopharmaceutical Products. Use of Optical Rotatory Dispersion and Circular Dichroism to Study Therapeutic Biomacromolecule Conformation. Use of Nuclear Magnetic Resonance for the Characterization of Biotherapeutic Products. Use of Chemical Probes for the Study of Protein Conformation. Use of Hydrogen Exchange in the Study of Biopharmaceutical Conformation. Use of Chemical Modifi cation for the Conformational Analysis of Biopharmaceuticals. Use of Immunology to Characterize Biopharmaceutical Conformation. Use of Limited Proteolysis to Study the Conformation of Proteins of Biotechnology Interest. Other Technologies for the Characterization of Conformational Change in Biopharmaceuticals. Development of an Experimental Approach for the Study of the Conformation of a Biological Therapeutic Product.