1st Edition

Basic Methods in Antibody Production and Characterization

ISBN 9780367202446
Published March 31, 2021 by CRC Press
284 Pages

USD $65.00

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Book Description

First published in 2001: Written for researchers and professionals in the fields of biomedical research, immunology, biochemistry, molecular biology, pathology, and biotechnology, Basic Methods in Antibody Production and Characterization uses a cookbook approach to presenting the methods for the production, characterization, and use of antibodies.
Antibodies described include polyclonal and monoclonal and those made by the phage display. Basic Methods in Antibody Production and Characterization focuses on specific aspects of antibodies and takes readers on a step-by-step process from antigen preparation to immunizations, adjuvants, screening, purification, storage, and applications.
Introductory material accompanies each chapter, giving readers a better understanding of the methods and applications. Ample references arm researchers with many applications pertaining to the particular technology.

Table of Contents

    1. Starting Out

    Delia R. Bethell and Gary C. Howard

    2. Animal Welfare And Regulatory Issues

    Sigmund T. Rich

    3. Antigens

    Donald H. Lewis

    4. Adjuvants

    Jory R. Baldridge and Michael J. Lacy

    5. Polyclonal Antibodies

    Eric S. Bean

    6. Monoclonal Antibody Production

    Sandy J. Stewart

    7. Screening Techniques

    Gamal E. Osman and Warren E. Ladiges

    8. Antibody Production By Ascites Growth

    Kristi R. Harkins

    9. Growth Of Cell Lines In Bioreactors

    Beverly J. Norris, Michael J. Gramer, and Mark Hirschel

    10. Phage Display

    Peter Amersdorfer and James D. Marks

    11. Antibody Purification Methods

    Kristi R. Harkins

    12. Molecular Characterization Of Immunoglobulin Genes

    Warren Ladiges and Gamal E. Osman

    13. Antibody Storage

    Kathryn Elwell and Delia R. Bethell

    14. Chemically Modifying Antibodies

    Kathryn Elwell and Gary C. Howard

    15. Western Blotting

    Lee Bendickson and Marit Nilsen-Hamilton

    16. Immunohistochemical Methods

    Kendrick J. Morrell, Neil M. Hand, and Nicholas R. Griffin

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    Gary C. Howard, Ph.D., is a senior scienfitic editor for the J. David Gladstone Institutes.

    Delia R. Bethell, Ph.D.,  is director of research with BioSeparations, Inc., in Tuscon, AZ.