First published in 2001: Written for researchers and professionals in the fields of biomedical research, immunology, biochemistry, molecular biology, pathology, and biotechnology, Basic Methods in Antibody Production and Characterization uses a cookbook approach to presenting the methods for the production, characterization, and use of antibodies.
Antibodies described include polyclonal and monoclonal and those made by the phage display. Basic Methods in Antibody Production and Characterization focuses on specific aspects of antibodies and takes readers on a step-by-step process from antigen preparation to immunizations, adjuvants, screening, purification, storage, and applications.
Introductory material accompanies each chapter, giving readers a better understanding of the methods and applications. Ample references arm researchers with many applications pertaining to the particular technology.
Table of Contents
1. Starting Out
Delia R. Bethell and Gary C. Howard
2. Animal Welfare And Regulatory Issues
Sigmund T. Rich
Donald H. Lewis
Jory R. Baldridge and Michael J. Lacy
5. Polyclonal Antibodies
Eric S. Bean
6. Monoclonal Antibody Production
Sandy J. Stewart
7. Screening Techniques
Gamal E. Osman and Warren E. Ladiges
8. Antibody Production By Ascites Growth
Kristi R. Harkins
9. Growth Of Cell Lines In Bioreactors
Beverly J. Norris, Michael J. Gramer, and Mark Hirschel
10. Phage Display
Peter Amersdorfer and James D. Marks
11. Antibody Purification Methods
Kristi R. Harkins
12. Molecular Characterization Of Immunoglobulin Genes
Warren Ladiges and Gamal E. Osman
13. Antibody Storage
Kathryn Elwell and Delia R. Bethell
14. Chemically Modifying Antibodies
Kathryn Elwell and Gary C. Howard
15. Western Blotting
Lee Bendickson and Marit Nilsen-Hamilton
16. Immunohistochemical Methods
Kendrick J. Morrell, Neil M. Hand, and Nicholas R. Griffin
Gary C. Howard, Ph.D., is a senior scienfitic editor for the J. David Gladstone Institutes.
Delia R. Bethell, Ph.D., is director of research with BioSeparations, Inc., in Tuscon, AZ.