Basic Plant Pathology Methods: 2nd Edition (Hardback) book cover

Basic Plant Pathology Methods

2nd Edition

By James B. Sinclair, Onkar Dev Dhingra

CRC Press

448 pages

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Hardback: 9780873716383
pub: 1995-04-05
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The Second Edition of this bestseller brings together basic plant pathology methods published in diverse and often abstract publications. The Second Edition is updated and expanded with numerous new figures, new culture media, and additional methods for working with a greater number of organisms. Methods are easy to use and eliminate the need to seek out original articles. This reference allows for easy identification of methods appropriate for specific problems and facilities. Scientific names of pathogens and some of their hosts are updated in this edition. The book also acts as a research source providing more than 1,800 literature citations.

The Second Edition includes chapters on the following:

  • Sterilization of culture apparatus and culture media

  • Culture of pathogens with detailed techniques for 61 fungi and selected bacteria

  • Long-term storage of plant pathogens

  • Detection and estimation of inoculum for 28 soilborne fungal pathogens and 5 bacterial genera-15 methods for airborne inoculum and 13 methods for seedborne pathogens

  • Establishment of disease and testing for disease resistance

  • Work with soil microorganisms

  • Fungicide evaluation

  • Biological control

  • Bright-field microscopy
  • Table of Contents

    Sterilization of Apparatus and Culture Media

    Heat Sterilization

    Gas Sterilization


    Culture of Pathogens

    Factors Affecting the Cultivation and Sporulation of Pathogenic Fungi

    Some Special Methods of Inducing Sporulation in Fungi

    Cultivation of Pathogens for Soil Infestation

    Other Sources of Inoculum

    Cultivation and Sporulation of Some Pathogens

    Long-Term Storage of Plant Pathogens

    Periodic Transfer

    Mineral Oil


    Storage in Water

    Storage in Silica Gel

    Storage in Soil


    Preservation in Liquid Nitrogen

    Lyophilization and Vacuum Drying

    Detection and Estimation of Inoculum

    Soilborne Pathogens

    Detection and Estimation of Inoculum in the Air

    Detection of Pathogens Associated with Seeds

    Establishment of Disease and Testing for Resistance

    Factors Influencing Disease Establishment

    Root Inoculation

    Stem Inoculation

    Leaf Inoculation

    Inoculation of Cereal Inflorescences

    Seed Inoculation

    Inoculation with Bacteria

    Inoculation of Detached Plant Parts

    Field Inoculation Methods

    Some Special Hints

    Soil Microorganisms

    Isolation and Enumeration of Soil Organisms

    Observation of Soil Microorganisms in Situ

    Physiological Processes as an Estimate of Biological Activity in Soil

    Spore Germination in Soil and Soil Fungistasis

    Survival of Plant Pathogens in Soil

    Mycelial Growth in Soil

    Collection of Root and Seed Exudates

    Artificial Rhizosphere or Spermosphere

    Fungicide Evaluation

    Spore Germination Test

    Tests Based on Growth on Organisms In Vitro

    Transfer-Bioassay Techniques

    Laboratory Testing for Soil Fungicides

    Greenhouse Testing of Foliar Fungicides

    Greenhouse Testing of Fungicides for Control of Soilborne Pathogens

    Planning Field Experiments

    Seed Treatment with Chemicals

    Biological Control

    Collection and Testing of Soil Samples for Antagonists

    Isolation of Antibiotic-Producing Organisms

    Isolation and Testing of Lytic Organisms

    Isolation and Testing of Mycoparasitic Organisms

    Spore Perforating Amoebae

    Isolation of Antagonists from Leaves

    Testing Antagonists for Biological Control

    Bright-Field Microscopy Techniques

    Mounting and Staining of Fungi for Microscopic Examination

    Staining of Fungal Nucelli

    Mounting Spores for Viewing from Different Positions

    Immobilization of Fungus Spores in Water Mounts for Microscopic Examination

    Staining of Bacteria

    Cements for Sealing Mounts

    Slide Culture Techniques and Preparation of Permanent Mounts

    Preparing Agar-Colony Mounts and Dry Reference Cultures

    Examination of Host-Parasite Relationship Using Bright-Field Microscopy


    Culture Media and Their Formulas

    Plant Nutrient Solutions

    Solutions to Maintain Constant Humidity in a Closed Atmosphere

    Drying Agents

    pH Indicator Dyes

    Approximate Figures for Concentrated Acids and Bases

    Preservation of Museum Specimens

    Collection, Preparation, and Mailing of Cultures and Specimens

    Glassware Cleaning Solutions


    About the Authors

    Sinclair\, James B.; Dhingra\, Onkar Dev

    Subject Categories

    BISAC Subject Codes/Headings:
    SCIENCE / Life Sciences / Botany