Biocalorimetry: Foundations and Contemporary Approaches, 1st Edition (Hardback) book cover


Foundations and Contemporary Approaches, 1st Edition

Edited by Margarida Bastos

CRC Press

416 pages | 115 B/W Illus.

Purchasing Options:$ = USD
Hardback: 9781482246650
pub: 2016-05-09
eBook (VitalSource) : 9780429171918
pub: 2016-06-22
from $28.98

FREE Standard Shipping!


Connecting past, present, and future instrument development and use, Biocalorimetry: Foundations and Contemporary Approaches explores biocalorimetry’s history, fundamentals, methodologies, and applications. Some of the most prominent calorimeter developers and users share invaluable personal accounts of discovery, discussing innovative techniques as well as special and original applications. Wide in scope, the book also covers calorimetry use on membranes, nucleic acids, and proteins and addresses both thermodynamics and kinetics.

The book begins with a look at the historical development of calorimeters needed for biological research. It then describes advanced approaches that use high-quality commercial calorimeters to study biochemical and other biological processes. It also shows how novel experimental designs and data analysis procedures are applied to proteins, DNA, membranes, and living matter.


"This is a highly specialized collection of articles mainly by European chemists, biochemists, and biologists. The article topics surround the history, theory, and application of calorimetry in biology. The measurement of heat absorbed and exuded by matter, due to changes in surrounding temperature, forms the science of relevance to characterize the materials; this can be related to their structures and properties. Historically, calories have been defined as units of heat energy. The focus of several chapters includes the problem of measuring the very small amounts of heat specific to biological matter, which requires very sensitive and sophisticated instruments. In four sections, the articles cover the history and methods of calorimetry, the use of differential scanning and isometric titration calorimetry to characterize specific membranes, the calorimetry of nucleic acids and proteins, and applications of calorimetry to other areas, such as clinical samples, enzymes, and pharmaceuticals. Every article is replete with references, graphs, and mathematical analysis. The index is useful.

Summing Up: Recommended. Graduate students, researchers, professionals."

—N. Sadanand, Central Connecticut State University, in the January 2017 issue of CHOICE

"Whether the reader is new to the area or already an experienced scientist, this book will serve as the ultimate reference in the field of biocalorimetry. The very pioneers that gave us the instrumentation and techniques cover the history and background of biocalorimetry. The ongoing research is described by current experts in different fields of biocalorimetry. Everything is there."

Arne Schön, Research Scientist, Department of Biology, Johns Hopkins University

"This book is a much-needed update on the field of biothermodynamics and biocalorimetry. It starts with historical and sometimes personal views from some of the pioneers of this field, followed by reviews on the state of the art of calorimetry application to the study of biomembranes, nucleic acids, and proteins, as well as biomedical applications. I think all newcomers should take the time to read the book from its beginning to grasp how the field started and until the end to have an idea on where it is expanding to."

Watson Loh, Professor of Physical Chemistry, Institute of Chemistry, University of Campinas

Table of Contents

Introduction: Historical and Methodological Context


Margarida Bastos

From Classical Thermochemistry to Monitoring of Living Organisms

Ingemar Wadsö

A Journey in the Land of Biocalorimetry: A Quest for Knowledge

Lee D. Hansen

An Odyssey in Calorimetric Development: From Past to Future

Jaak Suurkuusk, Malin Suurkuusk, and Peter Vikegard

Enzyme-Catalyzed Reactions

Robert N. Goldberg

Membrane Characterization and Partition to Membranes

Temperature-Induced and Isothermal Phase Transitions of Pure and Mixed Lipid Bilayer Membranes Studied by DSC and ITC

Alfred Blume

The Thermotropic Properties of Ceramides in Aqueous or Lipidic Environments

Alicia Alonso and Felix M. Goñi

Peptide–Membrane Interactions Studied by Isothermal Titration Calorimetry

Johannes Klingler and Sandro Keller

DSC Studies on the Modulation of Membrane Lipid Polymorphism and Domain Organization by Antimicrobial Peptides

Karl Lohner

Kinetics of the Interaction of Amphiphiles with Lipid Bilayers Using Isothermal Titration Calorimetry

Patrícia A.T. Martins and Maria João Moreno

Nucleic Acids and Proteins: Stability and Their Interactions with Ligands

Calorimetry of DNA: Energetic Basis of the Double Helix

Peter L. Privalov

Allostery and Cooperative Interactions in Proteins Assessed by Isothermal Titration Calorimetry

Adrian Velazquez-Campoy

Protein Stability in Crowded Environments

Michael Senske, Simon Ebbinghaus, and Christian Herrmann

Isothermal Titration Calorimetry and Fluorescent Thermal and Pressure Shift Assays in Protein–Ligand Interactions

Vytautas Petrauskas, Lina Baranauskiené, Asta Zubriené, and Daumantas Matulis

Joining Thermodynamics and Kinetics by kinITC

Philippe Dumas

Calorimetric Approaches to Studying Complex Protein Structure–Function–Stability Relationships in Conformational Diseases: The Case of Cystathionine β-Synthase Angel L. Pey, Tomas Majtan, and Jan P. Kraus

Calorimetry as a Tool in Applied Fields

An Account in Calorimetric Research: From Fundamentals to Pharmaceuticals

Patrick Connelly

Calorimetry for Monitoring Mixed Bacterial Populations and Biofilms

Simon Gaisford

Isothermal Microcalorimetry for the Investigation of Clinical Samples: Past and Present

Olivier Braissant, Gernot Bonkat, and Alexander Bachmann

Calorimetric Assays for Heterogeneous Enzyme Catalysis: Hydrolysis of Cellulose and Biomass

Peter Westh and Kim Borch


About the Editor

Margarida Bastos is an associate professor in the Department of Chemistry and Biochemistry at the University of Porto, where she leads the biocalorimetry research group. She is a reviewer for many international journals and is involved with European community evaluation of research projects and international mobility grants. Having calorimetry as her main expertise, she has also been using other biophysical techniques, such as small angle x-ray diffraction (SAXD), neutron scattering, fluorescence spectroscopy, microscopy (SEM, TEM, and confocal), to study antimicrobial peptide/membrane interactions, polymer/surfactant mixtures, hydrophobic and hydrophilic compounds, and hydration of biopolymers. She earned a PhD in thermochemistry from Lund University.

Subject Categories

BISAC Subject Codes/Headings:
MEDICAL / Biochemistry
MEDICAL / Pharmacology
SCIENCE / Physics