Biochemical Techniques in the Heart fully describes classical biochemical measurements of parameters involved in contraction and relaxation in the heart. This fully detailed guide tells you how to make preparations of sarcoplasmic reticulum, sarcolemma, and cardiomyocytes and how to measure sodium-calcium and sodium-hydrogen exchange. Two chapters explain the measurement of the important enzyme sodium-potassium ATPase.
This book examines the most widely used tools in experimental cardiology and provides you with the recipe-setting up the technique, procurement of equipment, sample data and calculations, problems and trouble shooting, adapting to other species, modifications, and applicability. Undoubtedly, this text will be a great asset to cardiovascular physiologists, pharmacologists, experimental cardiologists, and students of physiology and pharmacology.
Table of Contents
Preparation of Cardiac Sarcoplasmic Reticulum, J.J. Leddy and B.S. Tauna
The Isolation of Myocardial Sarcolemma, G.F. Tibbits
Measurement of Na+-Ca2+ Exchange, G.F. Tibbits
Measurement of Sodium/Potassium ATPase in Myocardium, V. Elimban, A. Lukas, and N.S. Dhalla
Molecular Assessment of Cardiac Sodium Pump Activity, V. Elimban, K. Kato, and N.S. Dhalla
Methods for Measuring Sodium-Hydrogen Exchange in the Heart, H. Massaeli and G.N. Pierce
Preparation of Cardiomyocytes, B. Rodrigues and D.L. Severson