1st Edition

Biofuel Production Biological Technologies and Methodologies

By Ramkrishna Sen, Shantonu Roy Copyright 2023
    156 Pages 25 B/W Illustrations
    by CRC Press

    Biofuels and bioenergy have emerged as an alternative option based on their sustainability, concomitant waste treatment, and site-specific flexibility. This book encompasses all the knowhow of different biofuel production processes through biological methods. It describes recent advancements in all major biofuel technologies such as biohydrogen, biomethane, bioethanol, syngas and so forth. Related protocols supported by schematic representation are included, encompassing comprehensive up-to-date scientific and technological information in biofuels and bioenergy.


    • Includes practical approaches focused on process design and analysis in biofuel production via biological routes
    • Discusses kinetic equations of different microbial systems
    • Provides comprehensive coverage of biochemical kinetics and equations related to biofuel process
    • Describes protocols for setting up of experiments for pertinent biofuel technologies
    • Emphasis on practical engineering approaches and experiments

    This book is aimed at researchers and graduate students in chemical, biochemical and bioprocess engineering, and biofuels.

    Chapter 1 Introduction to Biofuel and Bioenergy

    1.1 Introduction

    1.2 Renewable Energy Scenario

    1.3 Environmental and Ecological Impact


    Chapter 2 Dark Fermentative Hydrogen Production

    2.1 Introduction

    2.2 Microbial Perspective on Dark Fermentative Hydrogen-producing Microorganisms

    2.3 Biochemistry of Dark Fermentative Hydrogen Production

    2.4 Concept of Consortia Development

    2.5 Process Parameters Influencing Dark Fermentative Hydrogen Production

    2.6 Mathematical Models Expressing Substrate Conversion Efficiency and Microbial Growth

    2.7 Protocol for Biohydrogen Production



    Chapter 3 Biomethane Production Process

    3.1 Introduction

    3.2 Microbiology and Biochemistry of Methanogenesis

    3.3 Microbial Interactions

    3.4 Biochemistry of Biomethane Production

    3.5 Protocol for Biomethane Production Using Isolates



    Chapter 4 Bioethanol Production Process

    4.1 Introduction

    4.2 Feedstock for Bioethanol Production

    4.3 Protocol for Bioethanol Production



    Chapter 5 Biobutanol Production Process

    5.1 Introduction

    5.2 Characteristics of Butanol

    5.3 Technologies for Butanol Production

    5.4 Feedstocks for Biobutanol Production

    5.5 Purification and Separation of Butanol

    5.6 Protocol for Biobutanol Production



    Chapter 6 Algal Cultivation and Biodiesel Production from Its Biomass

    6.1 Introduction

    6.2 Present State of the Art of Photobioreactor (PBR) Development

    6.3 Protocol for Algal Cultivation



    Chapter 7 Bioelectricity Production Using Microbial Fuel Cell

    7.1 Introduction

    7.2 Classifications of Microbial Fuel Cells (MFC

    7.3 Microbiology and Biochemistry of Microbial Fuel Cells

    7.4 Biochemistry of Microbial Fuel Cell

    7.5 Methods for Understanding Performance of MFC

    7.6 Microbial Fuel Cell Design




    Ramkrishna Sen is presently Professor and Head, Department of Biotechnology; Chairperson, School of Bioscience; Chairperson, Central Research Facility (Life Science Division), Prof.-in-charge, Advanced Lab for Plant Genetic Engineering and Joint Faculty of the P K Sinha Center for Bioenergy & Renewables at IIT Kharagpur. He served as a Fulbright Visiting Professor in the Columbia University, New York, USA. Prof. Sen has been engaged in R&D activities in the areas of Energy, Environment, Water & Healthcare, with a major focus on Green Process & Product Development in Microalgal-&-Microbial Biorefinery models.

    Shantonu Roy is a Gold Medalist in Microbiology in B.Sc. from Vidyasagar University. He did M.Sc. in Biotechnology from the Jadavpur University, Kolkata. He then moved to the Indian Institute of Technology (IIT) Kharagpur, West Bengal, India where he pursued his Ph.D. He has been awarded with prestigious DST INSPIRED faculty and DST Early career award. He is among the top 20 scientist working in the field of biohydrogen in India according to DST Status Report Hydrogen and fuel cell, 2021. Currently, he is working as assistant professor in the School of Community Science and Technology at Indian Institute of Engineering Science and Technology Shibpur, West Bengal, India. His research interests focus on hydrogen production and methane production from thermophilic bacteria. He has developed expertise on metagenomic analysis of thermophilic mixed consortia, proteomics and strain improvement by mutagenesis.