Biophysical Chemistry explores the concepts of physical chemistry and molecular structure that underlie biochemical processes. Ideally suited for undergradate students and scientists with backgrounds in physics, chemistry or biology, it is also equally accessible to students and scientists in related fields as the book concisely describes the fundamental aspects of biophysical chemistry, and puts them into a biochemical context.
The book is organized in four parts, covering thermodynamics, kinetics, molecular structure and stability, and biophysical methods. Cross-references within and between these parts emphasize common themes and highlight recurrent principles. End of chapter problems illustrate the main points explored and their relevance for biochemistry, enabling students to apply their knowledge and to transfer it to laboratory projects.
- Connects principles of physical chemistry to biochemistry
- Emphasizes the role of organic reactions as tools for modification and manipulation of biomolecules
- Includes a comprehensive section on the theory of modern biophysical methods and their applications
Table of Contents
Thermodynamics. Systems and their surroundings. State functions and the laws of thermodynamics. Energetics and chemical equilibria. Thermodynamics of transport processes. Electrochemistry. Kinetics. Reaction velocities and rate laws. Integrated rate laws for uni and biomolecular reactions. Reaction types. Rate-limiting steps. Binding reactions: one-step and two-step binding. Steady-state (enzyme) kinetics. Complex reaction schemes and their analysis. Temperature dependence of rate constants. Principles of catalysis. Molecular structure and stability. Molecular structure and interactions. Proteins. Nucleic acids. Computational biology. Optical spectroscopy. Magnetic resonance. Solution scattering. X-ray crystallography. Imaging and microscopy. Force measurements. Transient kinetic methods. Molecular mass, size, and shape. Calorimetry. Prefixes, units, constants. Mathematical concepts used in this book.
Dagmar Klostermeier is of the University of Muenster, Germany.
Markus G. Rudolph is of the Pharma Research & Early Development Division in the Roche Group, Switzerland.
"Biophysical Chemistry by Klostermeier and Rudolph is an excellent up-to-date addition to current text book resources covering biophysical theory and practice. The book is well-structured into four main parts on Thermodynamics, Kinetics, Molecular Structure and Stability, and finally Methods. It is understandable and easy to read. The figures are spectacular, and the equations are well defined. The index is thorough, and the last Appendix briefly summarises the mathematical concepts used in the book, although some mathematical training would be required to derive the steps to reach some of the resulting equations. It was particularly pleasing to see sections on X-ray Free Electron Lasers and on the use of lipidic cubic phase for the crystallisation of membrane proteins, since these cutting edge approaches are at the forefront of structural biology research.
The layout of the book aids understanding by providing boxes which explain particular concepts and formalisms, while not interrupting the general flow. Overall the book is very comprehensive and will be appropriate as a companion and supplement to the first and second year courses in biophysics provided for our Molecular and Cellular Biochemistry undergraduates here at Oxford."
—Elspeth F. Garman, Professor of Molecular Biophysics, University of Oxford
"This is a superb book with something for everyone who works in any aspect of modern molecular biology. It integrates all major structural and biophysical techniques in a single volume describing them with sufficient rigor for serious physical scientists, but also provides sufficient clarity and practical examples such that those lacking interest or more formal training can still clearly appreciate what is going on. Every technique and most aspects of theory are beautifully illustrated with simple diagrams. The emphasis is on giving the user enough insight that they can decide whether a particular technique
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