Biophysics: Tools and Techniques, 1st Edition (Paperback) book cover

Biophysics

Tools and Techniques, 1st Edition

By Mark C. Leake

CRC Press

390 pages | 8 Color Illus. | 58 B/W Illus.

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pub: 2016-08-11
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Description

An Up-to-Date Toolbox for Probing Biology

Biophysics: Tools and Techniques covers the experimental and theoretical tools and techniques of biophysics. It addresses the purpose, science, and application of all physical science instrumentation and analysis methods used in current research labs.

The book first presents the historical background, concepts, and motivation for using a physical science toolbox to understand biology. It then familiarizes undergraduate students from the physical sciences with essential biological knowledge.

The text subsequently focuses on experimental biophysical techniques that primarily detect biological components or measure/control biological forces. The author describes the science and application of key tools used in imaging, detection, general quantitation, and biomolecular interaction studies, which span multiple length and time scales of biological processes both in the test tube and in the living organism.

Moving on to theoretical biophysics tools, the book presents computational and analytical mathematical methods for tackling challenging biological questions including exam-style questions at the end of each chapter as well as step-by-step solved exercises. It concludes with a discussion of the future of this exciting field.

Future innovators will need to be trained in multidisciplinary science to be successful in industry, academia, and government support agencies. Addressing this challenge, this textbook educates future leaders on the development and application of novel physical science approaches to solve complex problems linked to biological questions.

Features:

  • Provides the full, modern physical science toolbox of experimental and analytical techniques, such as bulk ensemble methods, single-molecule tools, and live-cell and test tube methods
  • Incorporates worked examples for the most popular physical science tools, including full diagrams and a summary of the science involved in the application of the tool
  • Reinforces the understanding of key concepts and biological questions

A solutions manual is available upon qualifying course adoption.

Table of Contents

Introduction

The motivation for biophysics

What do we mean by a "toolbox?"

Makeup of the subsequent chapters in this book

Once more, unto the breach

Summary points

Questions

References

Orientation for the bio-curious

Introduction: the material stuff of life

Architecture of organisms, tissues and cells, and the bits in between

The chemicals that make cells work

Cell processes

Physical quantities in biology

Summary points

Questions

References

Making light work in biology

Introduction

Basic UV-VIS-IR absorption, emission, and elastic light scattering methods

Light microscopy basics

Non-fluorescence microscopy

Fluorescence microscopy: the basics

Basic fluorescence microscopy illumination modes

Summary points

Questions

References

Making light work harder in biology

Introduction

Super-resolution microscopy

Förster resonance energy transfer (FRET)

Fluorescence correlation spectroscopy (FCS)

Light microscopy of deep samples

Advanced biophysical techniques using elastic light scattering

Tools using the inelastic scattering of light

Summary points

Questions

References

Detection and imaging tools that use non-optical waves

Introduction

Electron microscopy (EM)

X-ray tools

NMR and other radio frequency and microwave resonance spectroscopies

Tools that use gamma rays, radioisotope decay, and neutrons

Summary points

Questions

References

Forces

Introduction

Rheology and hydrodynamics tools

Optical force tools

Magnetic force methods

Scanning probe microscopy and force spectroscopy

Electrical force tools

Tools to mechanically probe cells and tissues

Summary points

Questions

References

Complementary experimental tools

Introduction

Bioconjugation

Model organisms

Molecular cloning

Making crystals

High-throughput techniques

Characterizing physical properties of biological samples in bulk

Biomedical physics tools

Summary points

Questions

References

Theoretical biophysics

Introduction

Molecular simulation methods

Mechanics of biopolymers

Reaction, diffusion, and flow

Advanced in silico analysis tools

Rigid-body mechanics

Summary points

Questions

References

Emerging biophysics techniques

Introduction

Systems biology: "systems biophysics"

Synthetic biology and bionanotechnology

Personalizing healthcare

Extending length and time scales to quantum and ecological biophysics

Summary points

Questions

References

Index

About the Author

Mark C. Leake is the chair of biological physics and the director of the Biological Physical Sciences Institute (BPSI) at the University of York. He heads an interdisciplinary research team in the field of single-molecule biophysics using cutting-edge biophotonics and state-of-the-art genetics. His work is highly cited, and he has won many fellowships and prizes.

Subject Categories

BISAC Subject Codes/Headings:
MED008000
MEDICAL / Biochemistry
MED009000
MEDICAL / Biotechnology
SCI055000
SCIENCE / Physics