An Up-to-Date Toolbox for Probing Biology
Biophysics: Tools and Techniques covers the experimental and theoretical tools and techniques of biophysics. It addresses the purpose, science, and application of all physical science instrumentation and analysis methods used in current research labs.
The book first presents the historical background, concepts, and motivation for using a physical science toolbox to understand biology. It then familiarizes undergraduate students from the physical sciences with essential biological knowledge.
The text subsequently focuses on experimental biophysical techniques that primarily detect biological components or measure/control biological forces. The author describes the science and application of key tools used in imaging, detection, general quantitation, and biomolecular interaction studies, which span multiple length and time scales of biological processes both in the test tube and in the living organism.
Moving on to theoretical biophysics tools, the book presents computational and analytical mathematical methods for tackling challenging biological questions including exam-style questions at the end of each chapter as well as step-by-step solved exercises. It concludes with a discussion of the future of this exciting field.
Future innovators will need to be trained in multidisciplinary science to be successful in industry, academia, and government support agencies. Addressing this challenge, this textbook educates future leaders on the development and application of novel physical science approaches to solve complex problems linked to biological questions.
- Provides the full, modern physical science toolbox of experimental and analytical techniques, such as bulk ensemble methods, single-molecule tools, and live-cell and test tube methods
- Incorporates worked examples for the most popular physical science tools, including full diagrams and a summary of the science involved in the application of the tool
- Reinforces the understanding of key concepts and biological questions
A solutions manual is available upon qualifying course adoption.
Table of Contents
The motivation for biophysics
What do we mean by a "toolbox?"
Makeup of the subsequent chapters in this book
Once more, unto the breach
Orientation for the bio-curious
Introduction: the material stuff of life
Architecture of organisms, tissues and cells, and the bits in between
The chemicals that make cells work
Physical quantities in biology
Making light work in biology
Basic UV-VIS-IR absorption, emission, and elastic light scattering methods
Light microscopy basics
Fluorescence microscopy: the basics
Basic fluorescence microscopy illumination modes
Making light work harder in biology
Förster resonance energy transfer (FRET)
Fluorescence correlation spectroscopy (FCS)
Light microscopy of deep samples
Advanced biophysical techniques using elastic light scattering
Tools using the inelastic scattering of light
Detection and imaging tools that use non-optical waves
Electron microscopy (EM)
NMR and other radio frequency and microwave resonance spectroscopies
Tools that use gamma rays, radioisotope decay, and neutrons
Rheology and hydrodynamics tools
Optical force tools
Magnetic force methods
Scanning probe microscopy and force spectroscopy
Electrical force tools
Tools to mechanically probe cells and tissues
Complementary experimental tools
Characterizing physical properties of biological samples in bulk
Biomedical physics tools
Molecular simulation methods
Mechanics of biopolymers
Reaction, diffusion, and flow
Advanced in silico analysis tools
Emerging biophysics techniques
Systems biology: "systems biophysics"
Synthetic biology and bionanotechnology
Extending length and time scales to quantum and ecological biophysics
Mark C. Leake is the chair of biological physics and the director of the Biological Physical Sciences Institute (BPSI) at the University of York. He heads an interdisciplinary research team in the field of single-molecule biophysics using cutting-edge biophotonics and state-of-the-art genetics. His work is highly cited, and he has won many fellowships and prizes.
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