This new edition features research from nearly 60 of the profession's most distinguished international authorities. Recognizing emerging developments in biopolymer systems research with fully updated and expanded chapters, the second edition discusses the biopolymer-based multilayer structures and their application in biosensors, the progress made in the understanding of protein behaviour at the air-water interface, experimental findings in ellipsometry and reflectometry, and recent developments concerning protein interfacial behaviour in microfabricated total analysis systems and microarrays. With over 3000 references, this is an essential reference for professionals and students in surface, pharmaceutical, colloid, polymer, and medicinal chemistry; chemical, formulation, and application engineering; and pharmacy.
Part I: Concepts 1. Macromolecular Adsorption: A Brief Introduction 2. Driving Forces for Protein Adsorption at Solid Surfaces 3. Thermodynamics of Adsorption of Amino Acids, Small Peptides, and Nucleic Acid Components on Silica Adsorbents 4. Quantitative Modeling of Protein Adsorption 5. Interfacial Behavior of Protein Mutants and Variants 6. Orientation and Activity of Immobilized Antibodies 7. Interactions between Surfaces Coated with Carbohydrates, Glycolipids, and Glycoproteins 8. Protein Adsorption Kinetics 9. Mobility of Biomolecules at Interfaces 10. Protein Adsorption in Relation to Solution Association and Aggregation 11. Mechanism of Interfacial Exchange Phenomena for Proteins Adsorbed at Solid-Liquid Interfaces 12. Interactions between Proteins and Surfactants at Solid Interfaces Marie Wahlgren 13. Toward Functionalized Polyelectrolyte Biofilms 14. Self-Assembly of Functional Protein Multilayers: From Planar Films to Microtemplate Encapsulation 15. Biological Activity of Adsorbed Proteins 16. Protein Interactions with Monolayers at the Air-Water Interface Part II: Methods 17. Local and Global Optical Spectroscopic Probes of Adsorbed Proteins 18. Proteins on Surfaces: Methodologies for Surface Preparation and Engineering Protein Function 19. Studies on the Conformation of Adsorbed Proteins with the Use of Nanoparticle Technology 20. Scanning Calorimetry in Probing the Structural Stability of Proteins at Interfaces 21. Ellipsometry and Reflectometry for Studying Protein Adsorption 22. Surface Plasmon Resonance Spectroscopies for Protein Binding Studies at Functionalized Surfaces 23. Neutron Reflection Study of Protein Adsorption at the Solid-Solution Interface 24. XPS, to F-SIMS, and MALDI-MS for Characterizing Adsorbed Protein Films Part III: Applications 25. Interaction of Proteins with Polymeric Synthetic Membranes 26. Protein Adsorption in Intravenous Drug Delivery 27. Control of Protein Adsorption in Solid-Phase Diagnostics 28. Protein Interfacial Behavior in Microfabricated Analysis Systems and Microarrays 29. Protein Adsorption in the Oral Environment 30. Adsorbed Biopolymers: Behavior in Food Applications