This book highlights that the availability of recombinant DNA techniques and the discovery of the Ti plasmid system have opened the plant kingdom to gene transfer experiments. It outlines biotechnology and biological techniques to elucidate interesting biological pathways unique to plants.
Part I: Biotechnology 1. Rabies Virus Glycoprotein Analogs: Biosynthesis in 2. Secretion of Human Interferons by Yeast 3. Multiple Point Mutations Affecting the Simian Virus 40 Enhancer 4. Human Insulin from Recombinant DNA Technology 5. Monoclonal Antibodies for Diagnosis of Infectious Diseases in Humans 6. Immunotoxins: A New Approach to Cancer Therapy 7. Protein Sequence Analysis: Automated Microsequencing 8. Antibodies That React with Predetermined Sites on Proteins 9. Protein Engineering 10. Prospects in Plant Genetic Engineering 11. Isolation of Agronomically Useful Mutants from Plant Cell Cultures 12. Genetic Transfer in Plants Through Interspecific Protoplast Fusion 13. Contributions of Conventional Plant Breeding to Food Production 14. Biotechnology of Forest Yield 15. Genetic Manipulation of Antibiotic-Producing Microorganisms 16. New Applications of Microbial Products 17. Bacterial, Viral, and Fungal Insecticides 18. Immobilized Enzymes and Cells as Practical Catalysts 19. Bioreactors: Design and Operation 20. Production of Feedstock Chemicals 21. Single-Cell Proteins Part II: Biological Frontiars 22. Monoclonal Antibodies Reveal the Structural Basis of Antibody Diversity 23. Genes of the Major Histocompatibility Complex in Mouse and Man 24. Studying Promoters and Terminators by Gene Fusion 25. Transcription of Class III Genes: Formation of Preinitiation Complexes 26. BK Viral Enhancer Element and a Human Cellular Homolog 27. Duplication of an Insertion Sequence During Transpositional Recombination 28. Translocations Among Antibody Genes in Human Cancer 29. Cellular Oncogenes and Multistep Carcinogenesis 30. Yeast RNA Polymerase II Genes: Isolation with Antibody Probes 31. Directed Mutagenesis of Dihydrofolate Reductase 32. Surface Molecules Identify Groups of Growing Axons 33. Modulation of Synapse Formation by Cyclic Adenosine Monophosphate 34. In Situ Hybridization to Study the Origin and Fate of Identified Neurons 35. Metallothionein-Human GH Fusion Genes Stimulate Growth of Mice 36. Introduction of Genetic Material into Plant Cells