Brain Microtubule Associated Proteins lays a firm foundation for understanding the brain microtubule associated proteins and how they contribute to the neurodegeneration of Alzheimer's disease. Few other reference works contain within a single source such a complete treatment of this topic. The authors cast a broad net over the field by devoting the first four chapters to each of the major brain microtubule associated proteins, MAP1A, MAP1B, MAP2 and tau. Completing this background material are chapters on the cellular role of these proteins in developing neurons and the sorting of tau to axons. The remaining chapters focus on tau - its kinases, its phosphatases, and other post-translational modifications such as ubiquitination and glycation.
The second part of the book takes a more practical approach and includes chapters on the purification of microtubule associated proteins, functional assays using gene-transfer experiments, immunofluorescence and video microscopic analysis.
This book is intended not only to be a reference source for expert laboratories but also to provide information which will allow a novice in the field to choose the best method for a particular purpose.
Part 1. General Aspects 1. Distinct Structural Features of the Microtubule Associated Protein 1 Family: MAP1A and MAP1B 2. Microtubule-associtaed Protein 1B (MAP1B): A Key Regulator in Neuritogenesis 3. Possible Roles for MAP2 in Neuronal Pathology 4. Tau: Structure and Function 5. MAPs in Growth Cones 6. Tau Protein Kinases 7. Tau Phosphatases 8. Ubiquitination of Tau as as Abnormal Protein 9. Glycation of Tau Protein. Implcations for the Aetiopathogenesis of Alzheimer's Disease 10. Sorting of Tau 11. How to Build a Paired Helical Filament 12. Modelling Prion-like Processing of Tau Protein in Alzheimer's Disease for Pharmaceutical Development Part 2. Practical Approaches 13. Expression and Purification of Tau for in vitro Studies 14. Immunofluorescence Population Assays for Studying MAP Function 15. Using Video Microscopy to Study Tau's Effects on the Dynamic Instability of Microtubule Assembly 16. Expression of MAP cDNAs in Eukaryotic Cells 17. Analysis of the Phosphorylation State of Tau in situ 18. Biochemical Isolation and Characterization of Paired Helical Filaments 19. Finding Biomedical Information on the Web