Calcium Signaling: 2nd Edition (Hardback) book cover

Calcium Signaling

2nd Edition

Edited by James W. Putney, Jr.

CRC Press

530 pages | 8 Color Illus. | 152 B/W Illus.

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pub: 2005-11-21
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The first edition of  James Putney’s Calcium Signaling offered readers a comprehensive view of the fascinating diversity of technologies that the new field of calcium signaling employed. And while that work is still regarded as a premier text on the basics of calcium signaling, progress has been so dramatic that an update is now required.

In Calcium Signaling, Second Edition, Putney focuses on those processes that generate calcium signals to compile the first comprehensive exploration of calcium signaling research from a methodological standpoint.

This new edition deals with methods for studying calcium from a variety of perspectives. Several chapters discuss calcium indicators and other tools, and look at microscopic and electrophysiological techniques, as well as other special methodological aspects of calcium signaling research. Other chapters examine the study of different systems, ranging from those found in yeast to those found in mammals, and several more are devoted to the cellular and molecular basis for calcium signaling, including explorations of receptors, calcium pumps, apoptosis, and drug delivery.

Once again, Putney has called upon top researchers from across the globe to contribute their expertise. Several new chapters have been added and in many cases, where chapters from the first edition were retained, new researchers were recruited to offer a fresh perspective.


As calcium signaling involves such a breadth of technical approaches and a wide range of applications, this work contains invaluable information for established researchers, as well as those graduate students and scientists just beginning to find a direction in cellular calcium signaling.


“… explores the molecular basis, as well as the complex aspects, of calcium signaling; employs an easy-to-follow format in describing numerous research techniques, along with the pros and cons of each approach; discusses the major intracellular and surface membrane calcium channel molecules and intracellular effectors in terms of their molecular nature and biophysical behavior; introduces the application of high-throughout technology, and the use of genetically tractable organisms such as C. elegans and yeast. … involves such a breadth of technical approaches and a wide range of applications; this work contains invaluable information for established researchers, as well as those graduate students and scientists just beginning to find a direction in cellular calcium signaling. ”

— In Anticancer Research, Vol. 26, 2006

"Dr. James Putney has done the subject a great service … . The texts are clear, well defined and excellently supported by references with an extensive index. … undoubtedly a very valuable and useful text."

— Geoffrey R. Dixon in Institute of Biology, Vol. 55 No.1, 2008

Table of Contents

Fluorescent Calcium Indicators Based on BAPTA; Martin Poenie

Fluorescent Indicators—Facts and Artifacts; Gary St. J. Bird and James W. Putney Jr.

Fluorescence Microplate-Based Techniques for the High-Throughput Assessment of Calcium Signaling:  The Highs and Lows for Calcium Researchers; Gregory R. Monteith and Gary St. J. Bird

Genetically Encoded Fluorescent Calcium Indicator Proteins; Atsushi Miyawaki, Takeharu Nagai, and Hideaki Mizuno

Targeted Aequorins; Andrea Prandini and Rosario Rizutto

Electrophysiological Recordings of Ca2• Currents; Anant B. Parekh

Molecular Biology of Ca2• Channels: Lessons from the TRP Superfamily; Veit Flockerzi, Thomas Aberle, Marcel Meissner, Christine Jung,  Stephan Philipp, and Ulrich Wissenbach

Genetic and Molecular Characterization of Ca2• and IP3 Signaling in the Nematode Caenorhabditis elegans; Ana Y. Estevez and Kevin Strange

Calcium Signaling Networks in Yeast; Kyle W. Cunningham

Nuclear Patch Clamp Electrophysiology of Inositol Trisphosphate Receptor Ca2• Release Channels; Don-On Daniel Mak, Carl White, Lucian Ionescu, and J. Kevin Foskett

Ryanodine Receptors; Xander H.T. Wehrens, Stephan E. Lehnart, and Andrew R. Marks

Methods in Cyclic ADP-Ribose and NAADP Research; Anthony J. Morgan, Grant C. Churchill, Roser Masgrau, Margarida Ruas, Lianne C. Davis, Richard A. Billington,  Sandip Patel, Michiko Yamasaki, Justyn M. Thomas, Armando A. Genazzani, and Antony Galione

Methods for Studying Calcium Pumps; Leonard Dode, Luc Raeymaekers, Ludwig Missiaen, Bente Vilsen, Jens P. Andersen, and Frank Wuytack

Measuring Single Cell and Subcellular Ca2• Signals; Sandip Patel, Lawrence D. Gaspers, Nicola Pierobon, Walson Metzger, and Andrew P. Thomas

Subcellular Compartmentalization of Calcium Signaling; Nicholas J. Dolman, Michael C. Ashby, Myoung K. Park, Ole H. Petersen, Alexei V. Tepikin

Apoptosis; Clark W. Distelhorst

Calcium Entry Channels and Drug Discovery; Su Li, Anne Dodge, Chris T. Poll, and Martin Gosling



Subject Categories

BISAC Subject Codes/Headings:
MEDICAL / Biochemistry
SCIENCE / Life Sciences / Cytology
SCIENCE / Research & Methodology
SCIENCE / Life Sciences / General