Chemistry of Protein and Nucleic Acid Cross-Linking and Conjugation  book cover
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Chemistry of Protein and Nucleic Acid Cross-Linking and Conjugation




ISBN 9780849374913
Published October 10, 2011 by CRC Press
622 Pages - 3 Color & 198 B/W Illustrations

 
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Book Description

Since the publication of the first edition of Chemistry of Protein Conjugation and Cross-Linking in 1991, new cross-linking reagents, notably multifunctional cross-linkers, have been developed and synthesized. The completion of the human genome project has opened a new area for studying nucleic acid and protein interactions using nucleic acid cross-linking reagents, and advances have also been made in the area of biosensors and microarray biochips for the detection and analysis of genes, proteins, and carbohydrates. In addition, developments in physical techniques with unprecedented sensitivity and resolution have facilitated the analysis of cross-linked products.

Updated to reflect the advances of the 21st century, this book offers:

  • An overview of the chemical principles underlying the processes of cross-linking and conjugation
  • A thorough list of cross-linking reagents published in the literature since the first edition, covering monofunctional, homobifunctional, heterobifunctional, multifunctional, and zero-length cross-linkers
  • Reviews of the use of these reagents in studying protein tertiary structures, geometric arrangements of subunits within complex proteins and nucleic acids, near-neighbor analysis, protein-to-protein or ligand–receptor interactions, and conformational changes of biomolecules
  • Discusses the application of immunoconjugation for immunoassays, immunotoxins for targeted therapy, microarray technology for analysis of various biomolecules, and solid state chemistry for immobilizations

Table of Contents

Overview of Protein Conjugation
References

Review of Protein and Nucleic Acid Chemistry
Introduction
Protein Composition
Protein Functional Groups
Nucleic Acid Chemistry
References

Reagents Targeted to Specific Functional Groups
Introduction.
Sulfhydryl Reagents
Amino Group–Specific Reagents
Reagents Directed toward Carboxyl Groups
Arginine-Specific Reagents.
Histidine-Selective Reagents
Methionine-Alkylating Reagents
Tryptophan-Specific Reagents
Serine-Modifying Reagents
References

How to Design and Choose Cross-Linking Reagents
Introduction
Use of Nucleophilic Reactions
Use of Electrophilic Reactions
Incorporating Group-Directed Reagents
Incorporating Photoactivatable Nonspecific Groups
Changing the Water Solubility of Cross-Linkers
Incorporating Special Characteristics in the Bridge Spacer
References

Homobifunctional Cross-Linking Reagents
Introduction
Amino Group–Directed Cross-Linkers
Sulfhydryl Group–Directed Cross-Linkers
Carboxyl Group–Directed Cross-Linking Agents
Phenolate and Imidazolyl Group–Directed Cross-Linking Reagents
Arginine Residue–Directed Cross-Linkers
Methionine Residue Cross-Linking Agent
Carbohydrate Moiety–Specific Reagents
Nondiscriminatory Photoactivatable Cross-linkers
Noncovalent Homobifunctional Cross-Linking Reagents
Nucleic Acid Cross-Linking Reagents
References

Heterobifunctional Cross-Linkers
Introduction
Group-Selective Heterobifunctional Reagents for Protein Cross- Linking
Protein-Photosensitive Heterobifunctional Cross-Linking Reagents
Noncovalent Immunoglobulin Cross-Linking System
Heterobifunctional Nucleic Acid Cross-Linking Reagents
References

Multifunctional Cross-Linking Reagents
Introduction
Trifunctional Cross-Linkers
Tetrafunctional Cross-Linkers
Multifunctional Cross-Linkers
Noncovalent Cross-Linkers
References

Monofunctional and Zero -Length Cross -Linking Reagents
Introduction
Monofunctional Cross-Linking Reagents
Zero-Length Cross-Linking Reagents
References

General Approaches for Chemical Cross-Linking
Introduction
Classification of Cross-Linking Procedures
General Conditions for Cross-Linking
Cross-Linking Protocols for Commonly Used Reagents
Cross-Linking Protocols Based on Biological Systems
Conditions for Cleavage of Cross-Linked Complexes
Reaction Complications
References

Analysis of Cross-Linked Products
Introduction
Techniques
References

Applications of Chemical Cross-Linking to the Study of Biological Macromolecules
Introduction
Determination of Tertiary Structures of Proteins
Determination of Quaternary Structures of Proteins
Determination of Protein–Protein Interactions
Detection of Protein Conformational Changes
Determination of Nucleic Acid Interaction and Nucleic Acid–Protein Interaction
Effects of Cross-Linking on Structural Stability and Biological Activity
References

Applications of Chemical Conjugation in the Preparation of Immunoconjugates and Immunogens
Introduction
Preparation of Immunoconjugates
Preparation of Immunogens
Characterization of Conjugation Methods
References

Application of Chemical Conjugation for the Preparation of Immunotoxins and Other Drug Conjugates for Targeting Therapeutics
Introduction
Targeting Agents and Toxins
Preparation of Therapeutic Conjugates
References

Application of Chemical Conjugation to Solid-State Chemistry
Introduction
Functionalities of Matrices
Protein Immobilization by Matrix Activation
Cross-Linking Reagents Commonly Used for Immobilization of Biomolecules
Immobilization by Cross-Linking through Carbohydrate Chains
Examples of Applications of Solid-Phase Immobilization Chemistry
References

Appendix A
: Amino Group–Directed Homobifunctional Cross-Linkers
Appendix B: Sulfhydryl Group– Directed Homobifunctional Cross-Linkers.
Appendix C: Phenolate- and Imidazolyl-Group-Directed Reagents: Bisdiazonium Precursors
Appendix D: Group Selective Heterobifunctional Cross-Linkers
Appendix E: Photoactivatable Heterobifunctional Cross-Linking Reagents
Index

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Author(s)

Biography

Shan S. Wong, Ph.D., recently retired from the National Institutes of Health, where he served as a scientific review administrator and a program officer. In the latter capacity, he oversaw scientific programs in the area of alternative and complementary medicine. Previously, he served as director of clinical chemistry at Hermann Hospital and Lyndon B. Johnson General Hospital in Houston, Texas, and as a faculty member at the University of Texas Health Science Center at Houston. Before joining the University of Texas, Dr. Wong was a full professor of chemistry at the University of Massachusetts at Lowell. In addition to teaching at the University of Massachusetts at Lowell, he also taught chemistry courses at Denison University, Granville, Ohio, and Ohio State University, Columbus.

Dr. Wong has published extensively in various scientific journals in the area of enzymology and clinical chemistry. He has received numerous honors and awards and has been active in various professional societies.

David M. Jameson, Ph.D., joined the Department of Cell and Molecular Biology at the John A. Burns School of Medicine at the University of Hawaii in 1989, where he is presently a full professor. Before moving to Hawaii he was on the faculty of the Pharmacology Department at the University of Texas Southwestern Medical School in Dallas.

Dr. Jameson received his BS in chemistry from Ohio State University in 1971 and his PhD in biochemistry from the University of Illinois at Urbana-Champaign in 1978. His thesis advisor was Gregorio Weber, who laid the foundations of modern fluorescence spectroscopy. Dr. Jameson carried out postdoctoral research at the Université Paris-Sud at Orsay, France before returning to the University of Illinois for a postdoctoral period in Gregorio Weber’s laboratory. In 1983, he joined the Pharmacology Department at the University of Texas Southwestern Medical Center at Dallas as an assistant professor. In 1989, he moved to the University of Hawaii.

Dr. Jameson’s primary research focus has always been the development and application of fluorescence approaches for the study of biomolecular interactions, in particular protein–protein and protein–ligand interactions. He has published extensively in this area (some 130 publications to date) and has received funding from the National Science Foundation, the American Heart Association, and the National Institutes of Health. He has also received the Established Investigator Award from the American Heart Association and the 2004 Gregorio Weber Award for Excellence in Fluorescence Theory and Application. He lectures at numerous fluorescence workshops around the world and is co-organizer of the International Weber Symposium on Innovative Fluorescence Methodologies in Biochemistry and Medicine held every three years in Hawaii.

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