Coherent Raman Scattering Microscopy  book cover
1st Edition

Coherent Raman Scattering Microscopy

ISBN 9781138199521
Published April 16, 2018 by CRC Press
610 Pages 284 Color & 284 B/W Illustrations

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Book Description

The First Book on CRS Microscopy

Compared to conventional Raman microscopy, coherent Raman scattering (CRS) allows label-free imaging of living cells and tissues at video rate by enhancing the weak Raman signal through nonlinear excitation. Edited by pioneers in the field and with contributions from a distinguished team of experts, Coherent Raman Scattering Microscopy explains how CRS can be used to obtain a point-by-point chemical map of live cells and tissues.

In color throughout, the book starts by establishing the foundation of CRS microscopy. It discusses the principles of nonlinear optical spectroscopy, particularly coherent Raman spectroscopy, and presents the theories of contrast mechanisms pertinent to CRS microscopy. The text then provides important technical aspects of CRS microscopy, including microscope construction, detection schemes, and data analyses. It concludes with a survey of applications that demonstrate how CRS microscopy has become a valuable tool in biomedicine.

Due to its label-free, noninvasive examinations of living cells and organisms, CRS microscopy has opened up exciting prospects in biology and medicine—from the mapping of 3D distributions of small drug molecules to identifying tumors in tissues. An in-depth exploration of the theories, technology, and applications, this book shows how CRS microscopy has impacted human health and will deepen our understanding of life processes in the future.

Table of Contents

Theory of Coherent Raman Scattering, Eric Olaf Potma and Shaul Mukamel
Coherent Raman Scattering under Tightly Focused Conditions, Eric Olaf Potma, Xiaoliang Sunney Xie, Andreas Volkmer, and Ji-Xin Cheng

Construction of a Coherent Raman Microscope, Brian G. Sarr and Xiaoliang Sunney Xie
Stimulated Raman Scattering Microscopy, Christian Freudiger and Xiaoliang Sunney Xie
Femtosecond versus Picosecond Pulses for Coherent Raman Microscopy, Mikhail N. Slipchenko, Delong Zhang, and Ji-Xin Cheng
Wide-Field CARS Microscopy, Alexander Jesacher, Gregor Thalhammer, Stefan Bernet, and Monika Ritsch-Marte
Vibrational Spectromicroscopy by Coupling Coherent Raman Imaging with Spontaneous Raman Spectral Analysis, Mikhail N. Slipchenko and Ji-Xin Cheng
Coherent Control in CARS, Jonathan M. Levitt, Ori Katz, and Yaron Silberberg
Fourier Transform CARS Microscopy, Jennifer P. Ogilvie
CRS with Alternative Beam Profiles, Varun Raghunathan, Hyunmin Kim, Stephan Stranick, and Eric Olaf Potma
Vibrational Phase Microscopy, Martin Jurna, Cees Otto, and Herman L. Offerhaus
Multiplex CARS Microscopy, James P.R. Day, Katrin F. Domke, Gianluca Rago, Erik M. Vartiainen, and Mischa Bonn
Interferometric Multiplex CARS, Sang-Hyun Lim
Photonic Crystal Fiber-Based Broadband CARS Microscopy, Marcus T. Cicerone, Young Jong Lee, Sapun H. Parekh, and Khaled A. Aamer
Multiplex Stimulated Raman Scattering Microscopy, Dan Fu and Xiaoliang Sunney Xie

Imaging Myelin Sheath Ex Vivo and In Vivo by CARS Microscopy, Yan Fu, Yunzhou (Sophia) Shi, and Ji-Xin Cheng
Imaging Lipid Metabolism in Caenorhabditis elegans and Other Model Organisms, Helen Fink, Christian Brackmann, and Annika Enejder
Lipid-Droplet Biology and Obesity-Related Health Risks, Thuc T. Le
White Matter Injury: Cellular-Level Myelin Damage Quantification in Live Animals, Erik Bélanger, F.P. Henry, R. Vallée, M.A. Randolph, I.E. Kochevar, J.M. Winograd, Charles P. Lin, and Daniel Côté
CARS Microscopy Study of Liquid Crystals, Heung-Shik Park and Oleg D. Lavrentovich
Live Cell Imaging by Multiplex CARS Microspectroscopy, Hideaki Kano
Coherent Raman Scattering Imaging of Drug Delivery Systems, Ling Tong and Ji-Xin Cheng
Applications of Stimulated Raman Scattering Microscopy, Christian Freudiger, Daniel A. Orringer, and Xiaoliang Sunney Xie
Applications of Coherent Anti-Stokes Raman Spectroscopy Imaging to Cardiovascular Diseases, Han-Wei Wang, Michael Sturek, and Ji-Xin Cheng
Applications of CARS Microscopy to Tissue Engineering, Annika Enejder and Christian Brackmann
Dietary Fat Absorption Visualized by CARS Microscopy, Kimberly K. Buhman


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Ji-Xin Cheng is a professor of biomedical engineering at Purdue University. He earned a PhD from the University of Science and Technology of China and completed postdoctoral research at the Hong Kong University of Science and Technology and Harvard University. He is a pioneer in the development and biomedical application of molecular vibration-based imaging tools.

Xiaoliang Sunney Xie is a Mallinckrodt Professor of Chemistry at Harvard University. He earned a PhD from the University of California-San Diego and completed postdoctoral research at the University of Chicago. He is well-known for his innovations in nonlinear Raman microscopy and his pioneering work in single-molecule biophysical chemistry, including enzyme dynamics and live cell gene expression.


"This book makes a seminal contribution to the field of biomedical imaging, and provides the reader with an excellent introduction and in-depth descriptions of the latest and greatest implementations of coherent Raman imaging technology—enough to jump start a new program or infuse new life into an existing one. The clear descriptions of a wide range of applications show how the methods are being used at present and indicate many different and varied future paths. This book is an excellent addition to the bookshelves of biomedical imaging practitioners and researchers in related fields, including surface analysis, process control, forensics, and others."
—David S. Moore, Los Alamos National Laboratory, Analytical and Bioanalytical Chemistry, 2013

"Interested readers can find an excellent mix of [optical platforms and applications given a solid theoretical approach] in this book, which is the first one dealing in a comprehensive and exhaustive way with CRS microscopy and related techniques. No one involved in optical microscopy and spectroscopy should miss this text, which is able to offer new exciting scenarios and, for sure, at least one new idea after reading."
—Alberto Diaspro, Italian Institute of Technology (IIT), Journal of Biomedical Optics, 2014