1st Edition
Drug Delivery with Targeted Nanoparticles In Vitro and In Vivo Evaluation Methods
Nanotechnology has the potential to change every part of our lives. Today, nanotechnology-based products are used in many areas, and one of the most important areas is drug delivery. Nanoparticulate drug delivery systems not only provide controlled delivery of drugs and improved drug solubility but also improve drug efficiency and reduce side effects via targeting mechanisms. However, compared with conventional drug delivery systems, few nanoparticle-based products are on the market and almost all are nontargeted or only passively targeted systems. In addition, obtaining targeted nanoparticle systems is quite complex and requires several evaluation mechanisms. This book discusses the production, characterization, regulation, and currently marketed targeted nanoparticle systems in a broad framework. It provides an overview of targeted nanoparticles’ (i) in vitro characterization, such as particle size, stability, ligand density, and type; (ii) in vivo behavior for different targeting areas, such as tumor, brain, and vagina; and (iii) current advances in this field, including clinical trials and regulation processes.
1. Particle Size Determination of Targeted Nanoparticles
Elif Bahar Yurttas, Tugba Gulsun, and Selma Sahin
2. Zeta Potential Determination of Targeted Nanoparticles
Melike Demirbolat, Zelihagül Değim, and İsmail Tuncer Değim
3. Stability of Targeted Nanoparticles
Susan D’Souza
4. Impact of PEGylation on Targeted Nanoparticulate Delivery
Yeşim Aktaş, Merve Çelik Tekeli, and Sedat Ünal
5. Ligands and Receptors for Targeted Delivery of Nanoparticles: Recent Updates and Challenges
Ankit Javia, Denish Bardoliwala, Mital Patel, and Ambikanandan Misra
6. Characterization of Biological Molecule–Loaded Nanostructures Using Circular Dichroism and Fourier Transform Infrared Spectroscopy
Ayhan Parlar, Prabir Kumar Kulabhusan, Hasan Kurt, Büşra Gürel, Milad Torabfam, Başak Özata, and Meral Yüce
7. Evaluation of Stimuli-Sensitive Nanoparticles in vitro
Naile Ozturk, Asli Kara, and Imran Vural
8. Analytical Techniques for Characterization of Nanodrugs
Asuman Doğanay, Nurdan Atιlgan, Bahar Koksel Ozgen, and Pelin Gurbetoglu
9. Cytotoxicity and Biological Compatibility
Ece Tavukcuoglu, Hamdullah Yanik, and Gunes Esendagli
10. Cellular Uptake and Transcytosis
Gozde Barin, Yakup Gultekin, Esra Pezik, Naile Ozturk, Asli Kara, and Imran Vural
11. Evaluation of 3D Cell Culture Models for Efficacy Determination of Anticancer Nanotherapeutics
Vineela Parvathaneni and Vivek Gupta
12. In vitro and in vivo Blood–Brain Barrier Models for the Evaluation of Drug Transport with Targeted Nanoparticles
Muge Yemisci
13. Sterility Evaluation of Targeted Nanoparticles
Melissa Vetten and Mary Gulumian
14. Evaluation of Pharmacokinetics and Biodistribution of Targeted Nanoparticles
Muge Ates, Nihan Izat, Fatma Kir, Tugba Gulsun, and Selma Sahin
15. Evaluation of the in vivo Preclinical Toxicity of Targeted Nanoparticles
Cengizhan Ceylan, Mücteba Eşref Tatlιpιnar, Sinem Tüccar, Gülden Zehra Omurtag, and Fatma Jülide Akbuğa
16. Transdermal Delivery of Targeted Nanoparticles and in vitro Evaluation
Ceyda Ekentok Atιcι, Aysun Çelik Soysal, and Ali Demir Sezer
17. In vivo and in vitro Evaluation of Nose-to-Brain Delivery of Nanoparticles
Tansel Çomoğlu and Sema Arısoy
18. Evaluation of Targeted Nanoparticles for Ocular Delivery
Naida Omerović, Edina Vranić, Jasmina Hadžiabdić, and Ognjenka Rahić
19. Vaginally Applied Nanocarriers and Their Characterizations
Fatmanur Tuğcu-Demiröz and Füsun Acartürk
20. Oral Administration of Nanoparticles and Approaches for Design, Evaluation, and State of the Art
Sedat Ünal and Erem Bilensoy
21. Drug Resistance Mechanisms and Strategies to Overcome Drug Resistance with Nanoparticulate Systems
Cem Varan, Gamze Varan, and Erem Bilensoy
22. Clinical Trials of Targeted Nanoparticulate Drug Delivery Systems
Adem Sahin, Cengizhan Ceylan, and Senem Ertan-Ahmed
23. Evaluation of Targeted Mesoporous Silica Nanoparticles
Hayrettin Tonbul
24. Evaluation of Targeted Liposomes
Serhat Kozlu
25. Prospects of Nanomedicine with Nanocrystal Technology
Onur Pinarbasli and Asuman Doganay
26. Characteristics of Marketed Nanopharmaceutics
Emine Şalva and Hayrettin Tonbul
27. Regulatory Guidelines of the US Food and Drug Administration and the European Medicines Agency for Actively Targeted Nanomedicines
Hacer Coşkun Çetintaş, Hayrettin Tonbul, Adem Şahin, and Yılmaz Çapan
Biography
Yilmaz Çapan is Professor and Head of the Pharmaceutical Technology Department, Faculty of Pharmacy, Hacettepe University, Turkey. He obtained his bachelor’s degree in pharmacy from Hacettepe University in 1972 and PhD with honors from the University of Lille, Faculty of Pharmacy, France, in 1978. He has authored more than 80 publications in prestigious, refereed pharmaceutical science journals, with a current citation number of more than 2000, and 3 books. Prof. Çapan is a member of the American Association of Pharmaceutical Scientists, the Association of Pharmaceutical Sciences of Ankara, the Turkish Pharmacists’ Association, and the Pharmacist’s Chamber of Ankara. His research interests include tabletting sustainedrelease oral solid dosage forms, bioadhesion, protein delivery from biodegradable microspheres, and brain drug targeting.
Adem Sahin is Assistant Professor at the Faculty of Pharmacy, Selçuk University, Turkey. He obtained his bachelor’s degree in pharmacy from Erciyes University, Turkey, in 2011 and PhD with honors from the Department of Pharmaceutical Technology, Hacettepe University, in 2017. He has been working in the pharmaceutical industry as a formulation specialist and a clinical trials executive and his research interests include brain drug delivery, cancer nanomedicine, and nanotechnology in drug delivery.
Hayrettin Tonbul is Assistant Professor at the Faculty of Pharmacy, Inönü University, Turkey. He obtained his bachelor’s degree in pharmacy from Near East University, Turkey, in 2015 and PhD with honors from the Department of Pharmaceutical Technology, Hacettepe University, in 2019. His research interests include controlled and targeted drug delivery, cancer nanomedicine, and nanotechnology in drug delivery.
“Jenny Stanford continues its great job on providing first-class information on drug delivery and nanomedicine by publishing this new book. Twenty-seven chapters in this book written by the leading scientists in the field cover multiple topics—from nanoparticle properties and characterization to regulatory issues, and from targeted nanoparticles to stimuli-sensitive ones. Very useful reading for many in the field.”
Prof. Vladimir Torchilin
Northeastern University, USA“This book is an essential reading and reference for all those interested in the design, characterization, and evaluation of nanoparticulate drug delivery systems. The authors have made an incredible effort to summarize and highlight in a single work the basic information needed to understand the physicochemical, pharmacotechnical, biological, clinical, and regulatory aspects related to the development of nanotherapeutics.”
Prof. Juan M. Irache
Universidad de Navarra, Spain
