Enzymology of Complex Glycans
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In all living systems various polyglycans exist having essential functions that are closely linked to glycan structures. All structural features of glycans are mediated by kinetic properties of glycan synthesizing enzymes. Often, glycan-related enzymes non-covalently bind to the carbohydrate substrate(s) by regions different from the active site. Removal of these binding sites often strongly affect carbohydrate specifics of the enzyme. This book describes recent progress in polyglycan-related enzymology. It covers kinetic features of enzymes and reactions, metabolic paths, the reversible formation of glycan-related enzyme complexes, and procedures used for structural analyses of polyglycans.
Table of Contents
Polyglycans and Related Enzymes. Evolutionary Aspects of Starch and Glycogen Metabolism. Principles of Polyglycan-Related Enzymes. Methods of Structural Analyses of Polyglycans. Metabolic Paths. Biosynthesis and Degradation of Starch. Turnover of Glycogen. Initiation of Starch and Glycogen. Reversible Modifications of Starch. Reversible Modifications of Glycogen. Non- Classical Protein Functions in Starch. Non-Classical Protein Functions in Glycogen. Biosynthesis and Modification of Cell Wall Materials. Polyglycan Structures. Second Harmonic Generation (SHG) Analyses of Starch Granules. Structural Analyses of Glycogen. In-situ Analyses of Cell Wall Materials.
Martin Steup, Felix Nitschke