Experimental Cell Biology of Taste and Olfaction: Current Techniques and Protocols, 1st Edition (Hardback) book cover

Experimental Cell Biology of Taste and Olfaction

Current Techniques and Protocols, 1st Edition

By Andrew I. Spielman, Joseph G. Brand

CRC Press

464 pages

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Hardback: 9780849376450
pub: 1995-07-12
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Experimental Cell Biology of Taste and Olfaction examines and adapts methods from a variety of established fields, such as neurophysiology, receptor biochemistry and cellular imaging to provide comprehensive coverage of current techniques and protocols in chemosensory cell biology. Written for both newcomers and established scientists, this volume offers numerous tips for problem solving and suggests ways to avoid the most common, and costly, mistakes made by researchers.

This book covers general aspects such as tissue collection and preparation, as well as specific, up-to-date methods used in taste and olfactory morphology, immunology, biochemistry, biophysics, electrophysiology and molecular biology. The explosion of knowledge and the increased interest in these areas make this book an important reference work for all scientists, students, and teachers in this and related fields

Table of Contents

General Methods in Chemosensory Research

Euthanasia, I. Ramirez

Collection of Taste Tissue from Fish and Amphibians, J. H. Teeter

Collection of Taste Tissue from Mammals, A. I. Spielman and J. G. Brand

Insects: Isolation of Olfactory Sensilla and Collection of Sensillum Lymph, G. Ziegelberger

Surgical Anatomy of Olfactory Organ and Tissue Collection Methods in Vertebrates, T. Kurahashi and D. Restrepo

Collection of Human Olfactory Tissue, L. D. Lowry and E. A. Pribitkin

Surgical Removal of the Vomeronasal Organ and Its Verification, C. J. Wysocki and L. M. Wysocki

Denervation and Reinnervation of the Tongue, B. Oakley

The Lingual Slice Preparation, Isolated Taste Cells, and Dye Injections in Mudpuppy, A. Bigiani, D. A. Ewald, and S. D. Roper

Cell Dissociation Methods for Patch Clamp Electrophysiological Studies of Vertebrate Olfactory Receptor Neurons, A. H. Kahn and S. Firestein

Tissue Culture of Rat Taste Buds, C. Ruiz, M. McPheeters, and S. C. Kinnamon

Olfactory Receptor Neuronal Cultures, G. V. Ronnett

Methods for Airborne and Aqueous Stimulus Delivery in Olfactory Research, J. S. Kauer and S. Firestein

Imaging Methods in Chemosensory Research

Videomicroscopy and Image Processing, I. J. Miller and S. L. Hayes

Transmission Electron Microscopy of Gustatory Epithelium, H. H. Crowley and J. C. Kinnamon

Scanning and Transmission Electron Microscopy of Olfactory Epithelia, B. Ph. M. Menco

Analysis of Taste Bud Ultrastructure by Serial Sectioning and Three-Dimensional Reconstruction, S. M. Royer and J. C. Kinnamon

Immunohistochemistry, C. DellaCorte

IP3 Autoradiography of Taste Receptor Cells, P. A. Hwang

In Situ Hybridization, H. Schmale and M. Behrens

Biochemical Methods in Chemosensory Research

Membrane Preparation and Subcellular Fractionation, D. L. Kalinoski

Preparation of Olfactory Cilia, R. R. H. Anholt

The Use of the Spiny Lobster as a Tool for the Study of Chemosensory Biochemistry, H. G. Trapido-Rosenthal

Receptor Binding and Solubilization, D. L. Kalinoski

Western Blotting, R. Bruch and Fe. C. Abogadie

Approaches for Monitoring Rapid Kinetics of Second Messenger Signaling, E. Tareilus, I. Boekhoff, A.I. Spielman, and H. Breer

Second Messenger Assays, A. I. Spielman, H. Nagai, G. Sunavala, M. Dasso, T. Huque, and J. G. Brand

Electrophysiological and Biophysical Methods in Chemosensory Research

General Methods

Reconstitution of Chemoreceptor Proteins in Planar Lipid Bilayers, A. M. Feigin, M. Zviman, and J. H. Teeter

Data Acquisition in Electrophysiology, V. E. Dionne

Whole Nerve and Single Unit Recordings

Taste Nerve Recording from a Voltage-Clamped Receptive Field, J. A. DeSimone, G. Heck, and Q. Ye

Whole Nerve and Single Fiber Recordings in Frog, T. Miyamoto and T. Sato

Single Unit Electrophysiological Recordings from Crustacean Chemoreceptor Neurons, C. D. Derby

Olfactory and Taste Recordings in Fish, J. Caprio

Taste Nerve Recordings in Rodents, M. E. Frank

Trigeminal Nerve Recordings in Rodents, B. P. Bryant

Whole Nerve and Single Fiber Recordings in Non-Human Primates, G. Hellekant and T. W. Roberts

Electrophysiology of the Vomeronasal Organ and Accessory Olfactory System, M. Meredith


Patch-Clamping of Taste Cells in the Mudpuppy, T. A. Cummings and S. C. Kinnamon

Patch-Clamping Recording from Taste Receptor Cells of Channel Catfish and Larval Tiger Salamander, J. H. Teeter

Patch-Clamping of Taste Cells in Hamster and Rat, T. A. Gilbertson

Patch-Clamping of Taste Cells in Mouse, J. F. MacDonald, I. Mody, M. W. Salter, and A. I. Spielman

Loose-Patch Recording from Taste Buds in Situ, B. Lindemann

Patch-Clamping Lobster Olfactory Receptor Cells, B. W. Ache, D. A. Fadool, and A. Zhainazarov

Patch-Clamping of Whole Olfactory Cilia, S. J. Kleene

Patch-Clamping Techniques for Vertebrate Olfactory Receptor Cells, G. Lowe, T. Nakamura, and G. H. Gold

Single Unit and Electroantennogram Recordings in Insect Olfactory Organs, K.-E. Kaissling

Patch-Clamping of Olfactory Cells in Insects, F. Zufall

Imaging of Intracellular Calcium in Chemosensory Receptor Cells, D. Restrepo, M. Zviman, and N. E. Rawson

Videorate Imaging of Potentiometric Dyes in Olfactory Research, J. S. Kauer

Methods to Study Flow Dynamics in Olfaction, P. W. Scherer, K. Keyhani, I. Hahn, and M. Mozell

Applications of Molecular Biology to Chemosensory Research

Cloning of Gustatory cDNAs and Analysis of Their Pattern of Expression, P. J. McKinnon, S. K. McLaughlin, and R. F. Margolskee

Cloning of Odorant Receptors, E. R. Liman and L. B. Buck

List of Critical References for Techniques Not Covered

Subject Categories

BISAC Subject Codes/Headings:
SCIENCE / Life Sciences / Cytology
SCIENCE / Life Sciences / Biology / Molecular Biology
SCIENCE / Life Sciences / General
SCIENCE / Life Sciences / Neuroscience