Forensic DNA Profiling: A Practical Guide to Assigning Likelihood Ratios, 1st Edition (Hardback) book cover

Forensic DNA Profiling

A Practical Guide to Assigning Likelihood Ratios, 1st Edition

By Jo-Anne Bright, Michael Coble

CRC Press

242 pages

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Hardback: 9780367029029
pub: 2019-12-18
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Description

DNA testing and its forensic analysis are recognized as the “gold standard” in forensic identification science methods. However, there is a great need for a hands-on step-by-step guide to teach the forensic DNA community how to interpret DNA mixtures, how to assign a likelihood ratio, and how to use the subsequent likelihood ratio when reporting interpretation conclusions.

Forensic DNA Profiling: A Practical Guide to Assigning Likelihood Ratios will provide a roadmap for labs all over the world and the next generation of analysts who need this foundational understanding. The techniques used in forensic DNA analysis are based upon the accepted principles of molecular biology. The interpretation of a good quality DNA profile generated from a crime scene stain from a single source donor provides an unambiguous result when using the most modern forensic DNA methods. Unfortunately, many crime scene profiles however are not single source. They are described as mixed since they contain DNA from two or more individuals.

Interpretation of DNA mixtures represents one of the greatest challenges to the forensic DNA analyst.

As such, the book introduces terms used to describe DNA profiles and profile interpretation. Chapters explains DNA extraction methods, the Polymerase Chain Reaction (PCR), Capillary Electrophoresis (CE), likelihood ratios (LRs) and their interpretation, and population genetic models—including Mendelian inheritance and Hardy-Weinberg Equilibrium. It is important that analysts understand how LRs are generated in a probabilistic framework, ideally with an appreciation of both semi-continuous and fully-continuous probabilistic approaches.

Key features:

• The first book to focus entirely on DNA mixtures and the complexities involved with interpreting the results

• Takes a hands-on approach offering theory with worked examples and exercises to be easily understood and implementable by laboratory personnel

• New methods, heretofore unpublished previously, provide a means to innovate deconvoluting a mixed DNA profile, assign an LR, and appropriately report the weight of evidence

• Includes a chapter on assigning LRs for close relatives (i.e., “It’s not me, it was my brother”), and discusses strategies for the validation of probabilistic genotyping software.

Forensic DNA Profiling fills the void for labs unfamiliar with LRs, and moving to probabilistic solutions, and for labs already familiar with LRs, but wishing to understand how they are calculated in more detail. The book will be a welcome read for lab professionals and technicians, students, and legal professionals seeking to understand and apply the techniques covered.

Table of Contents

1: Binary Mixture Interpretation Essentials

2: Laws of Probability and Bayes Rule

3: Heterozygote Single Source LR and Population Genetics Models

4: Two-Person Mixtures

5: Theory and Probability of dropout Pr(D), Probability of drop-in Pr(C)

6: Concept of weights, Pr(O|Sj), MCMC, and Q allele

7: Best Practices, Specificity and SConsiderations on validation of Probabilistic genotyping softwareensitivity Experiments, and Reproducibility

About the Authors

Jo-Anne Bright has an MSc and PhD in Forensic Science from the University of Auckland. She is a Senior Science Leader at the Institute of Environmental Science and Research Limited, in Auckland, New Zealand where she has worked since 1999. She has 20 years’ experience in forensic casework, quality management, and research. She has over 70 publications in the area of forensic DNA analysis and interpretation. Jo is a co-developer of the DNA profile interpretation software STRmix™ and has undertaken many presentations and workshops on DNA profile interpretation in Australasia, Asia, the United States and Europe.

Michael D. Coble is an Associate Professor and the Associate Director of the Center for Human Identification at the University of North Texas Health Science Center in Fort Worth, Texas. Dr. Coble received his Master’s Degree in Forensic Science and his PhD in Genetics from The George Washington University. He is a Fellow of the American Academy of Forensic Sciences and a member of the International Society for Forensic Genetics. He serves as a member of the OSAC Biological Data Interpretation and Reporting Committee and is an invited guest at the Scientific Working Group on DNA Analysis Methods (SWGDAM). He is a co-editor of the Forensic Biology subject area of WIREs Forensic Science journal and is a member of the editorial board of Forensic Science International: Genetics.

Subject Categories

BISAC Subject Codes/Headings:
LAW041000
LAW / Forensic Science
MED107000
MEDICAL / Genetics
SCI029000
SCIENCE / Life Sciences / Genetics & Genomics