Gap junction channels are a group of intercellular channels expressed in tissues and organs to synchronize many physiological processes. A gap junction channel is formed by the docking of two hemichannels, and each hemichannel is a hexamer of connexins. The field of gap junction channel and hemichannel research has recently exploded and became one of the most active areas of cell biology. Numerous novel approaches and techniques have been developed, but there is no single book dedicated to the unique techniques and protocols employed for the research on these large pore channels. This book fills the gap and focuses on protocols, approaches and reviews of gap junction channels and connexin hemichannels. It will be a useful reference for graduate students, postdoctoral fellows and researchers. Anyone with an interest in gap junction channels and hemichannels will need this summary of state-of-the-art techniques and protocols.
Table of Contents
Immunofluorescence: Applications for Analysis of Connexin Distribution and Trafficking Sandra A. Murray and T.I. Shakespeare
Imaging gap junctions in living cells
Matthias M. Falk, Charles G. fisher , Rachael, M Kells Andrew, Tia J Kowal
Fluorescence Recovery After Photobleaching (FRAP) for study of gap junction Nexus macromolecular organization
David C Spray, Randy F. Stout, Jr.
Patch clamp analysis of gap junction channel properties
Donglin Bai, and John A. Cameron
What do you need to measure gap junctional permselectivity
Jose Ek Vitorin
Recording gap junction mediated synaptic transmission in vivo at mixed synapses on the goldfish Mauthner cells
Alberto Pereda, Roger Cachope, Sebastian Curti
Assessing connexin hemichannel function during ischemic injury and reperfusion
Yeri Kim, Colin Green
Whole-cell patch-clamp recordings of unitary Cx43 hemichannel currents
Nan Wang, Alessio Lissoni, Karin R Sipido, Luc Laybaert, Maarten De Smedt
Functional characterization of connexin hemichannels using Xenopus oocytes and the two-electrode voltage clamp technique
Jorge E. Contreras, JMV Capuccino
Functional assays of purified connexin hemichannels
Guillermo A Altenberg, Mohamed Kreir
Methods to determinate formation of heteromeric hemichannels
Agustin D. Martínez, Oscar Jara, Ricardo Ceriani, Jaime Maripillan, Paula Mujica, Isaac E Garcia
Methods to examine the role of gap junction and pannexin channels in HIV infection
Eliseo A. Eugenin, Courtney A. Veilleux
Donglin Bai received his Ph.D. from University of Cambridge, U.K. (1994). He then moved to Canada and worked on neurotransmitter receptors (GABA, NMDA, AMPA, noradrenaline, angiotensin) in Loeb Research Institute (Ottawa), Department of Physiology (Toronto) and Samuel Lunenfeld Research Institute (Toronto) as a postdoctoral fellow. In 2002 he was recruited as an Assistant Professor at Department of Physiology and Pharmacology, the University of Western Ontario. He is currently a tenured Associate Professor in the same university. His current research interests are on the physiology of gap junction channels, including gap junction channel docking, single channel conductance and gating properties. He is also interested in revealing how gap junction gene mutants linking to human diseases (cardiac arrhythmias, hypomyelination, cataracts, deafness and skin diseases) and developing strategies of rescuing the mutants. He received grants support from the following Canadian funding agencies: CIHR, CRC, NSERC, HSFC and ERA.
Dr. Juan C. Sáez received his PhD in neuroscience from Albert Einstein College of Medicine (AECOM), New York, New York, in 1986. He stayed for one year as an instructor in the Department of Neuroscience of AECOM, and then, he became an assistant professor in the same department. In 1993, he joined the Physiology Department of the Pontificia Universidad Católica de Chile, Santiago, Chile, where he has been a professor since 2003. His current research interest is understanding the regulation and the function of connexin- and pannexin-based channels in different cell types, including the cells of the nervous system, the immune system, and the gastrointestinal system and, more recently, on skeletal muscles. He has also characterized biophysical features of the mentioned channels. Recently, he has also used protocols for identifying highly selective inhibitors of connexin hemichannels without an effect on gap junction channels and with potent anti-inflammatory activity to treat chronic diseases. He has been continuously funded through the National Institutes of Health and different foundations of the Chilean government.