Maximizing the potential of RNA interference in functional genomics - as well as in the development of therapeutics - continues to be at the forefront of biomedical research. Unlike journal articles, Gene Silencing by RNA Interference: Technology and Application combines essential background to the RNAi field with practical techniques designed by r
Gene silencing by RNA interference and the role of small interfering RNAs. Basics of siRNA design and chemical synthesis. Oligonucleotide scanning arrays in the design of small interfering RNAs. siRNA production by in vitro transcription. Production of siRNAs with the application of deoxyribozymes. Production of siRNA in vitro by enzymatic digestion of double-stranded RNA. Plasmid-mediated intra cellular expression of siRNAs. Lentiviral vector-mediated delivery of si/shRNA. Exogenous siRNA delivery: protocols for optimising delivery to cells. RNAi in Drosophila cell cultures RNAi in Caenorhabditis elegans. Delivery of RNAi reagents in C. elegans by microinjection. RNAi in Xenopus laevis oocytes. RNAi in Xenopus embryos. An RNAi-based genomic library for forward genetics in the African trypanosome. RNAi in the malaria parasite Plasmodium. Silencing the expression of cysteine proteases (falcipains) in Plasmodium falciparum using RNA interference approaches. RNAi in transgenic animal models. RNA interference in mouse model. Virus-induced RNA silencing in plants. RNA silencing in plants - biolistic delivery of RNAi reagents. Agro-infiltration: a versatile tool for RNAi studies in plant.