This book examines the revolutionary new technology of gene targeting, from its history to its potential application for the genetic treatment of human diseases. It begins with an introduction to the field and a description of the whole gene targeting spectrum. It continues with discussions in the following areas: The molecular mechanisms of the homologous recombination reactions lying behind gene targeting technologyThe application of gene targeting approaches to the problems of tripanosomatide biology and etiopathologyThe concept of gene targeting on plant genes and its impact on plant genetic engineeringThe current knowledge on mammalian development and physiology and its relationship to gene targeting technologyThe application of gene targeting technology, as well as other genetic techniques, to the production of animal models for human diseasesThe enzymatic complexes and components participating in natural bacterial DNA recombination and their potential application to gene targeting technologyThe potential application of gene targeting for the genetic treatment of human diseases
Table of Contents
Gene Targeting, W.M. Bertling.
Molecular Mechanisms of Homologous Recombination, A.S. Waldman.
Gene Targeting in Tripanosomatides, A.K. Cruz.
Gene Targeting in Plants, R. Offringa and P. Hooykaas.
Gene Targeting in Mammalian Development and Physiology, T. Lufkin.
Animal Models of Human Genetic Disease, J.-L. Guenet.
Homologous Recombination Proteins and Their Potential Applications in Gene Targeting Technology, S.C. Kowalczykowski and D.A. Zarling.
Gene Targeting in Human Gene Therapy, M.A. Vega.
BISAC Subject Codes/Headings:
- MEDICAL / Pharmacology
- SCIENCE / Life Sciences / Genetics & Genomics