Written by experts in the field, this title provides a comprehensive source of information on DNA sequencing and mapping, the newest technologiy and procedures in areas such as radiation hybrid mapping, FISH and specialized sequencing techniques. It also covers genomic sequence software and sequence databases. This book is an essential guide for anyone involved in DNA sequencing and mapping.
Table of Contents
1. Parameters of a new genome. 2. Radiation hybrid mapping - A whole chromosome approach. 3. Construction of large-insert bacterial clone libraries. 4. The use of YACs in mapping and sequencing. 5. Fluorescence In Situ Hybridization, Flow-sorting and Related Technologies. 6. Assembling Physical Maps and Sequence Clone Selection. 7. FPC: A Software Package for Physical Maps. 8. Mapping Pericentromeric Regions. 9. Cloning, mapping, and sequencing telomeres. 10. Shotgun Sequencing. 11. Finishing Genomic Sequence and Dealing with Problem Sequences. 12. Software for Genomic Sequencing. 13. Annotating Mammalian Genome Sequence. 14. Sequence Databases.