Methodology and Clinical Aspects
First published in 1982, this two-volume set provides the reader with insightful knowledge of HLA Typing and the different methods in which this can be undertaken. In this volume, we are presented with inside knowledge of the variety of HLA typing methods and the circumstances in which they are necessary.
Table of Contents
1. Separation Techniques for Mononuclear Blood Cells. 2. The HLA System and Cross-Reactivity. 3. New Aspects of HLA Serology and Biochemistry Defined Using Monoclonal Antibodies. 4. Lysostrip, F(ab’) ₂ Blocking. 5. Screening for HLA Sera. 6. Planned Immunization for the Production of HLA Alloantibodies. 7. Operationally Specific Xenoantisera to Allospecificities of the HLA Antigenic System. 8. Antigenic Constitution of HLA-A, B, C, Molecules: Immunochemical Analysis with Monoclonal Antibodies. 9. Technical Aspects and Clinical Relevance of HLA Typing and the Crossmatch Test in Kidney Transplantation. 10. Separation of B Lymphocytes and HLA-DR Typing. 11. Analysis of Kidney Transplant Outcome in relation to HLA-A, -B Matching. Presentation of a Multicenter Study from Scandinavia. 12. Histocompatibility Matching in Renal Transplantation. 13. HLA and Marrow Transplantation. Index.
1. Complement Fixation Tests in HLA Typing. 2. ADCC in Histocompatibility and Clinical Medicine. 3. HLA Typing of Cells in Long-Term Culture. 4. Granulocyte and Monocyte Antigens and Antibodies. 5. Data Analysis of Cellular Typing. 6. HLA-D Typing by MLC with Homozygous Typing Cells. 7. Primed Lymphocyte Typing (PLT): Application in Typing for HLA-D Locus Determinants. 8. Cell Mediated Lympholysis: A One Step Microplate Technique. 9. The Fluorochromasia Cell Mediated Lympholysis Assay (CML). 10. HLA-A, B Restriction of Cytotoxic T Cells. 11. Histocompatibility Typing by Polyclonal and Cloned Human Cytotoxic T Lymphocytes. 12. Involvement of HLA Cell Membrane Molecules in T Cell Immune Responses: Immunobiological and Clinical Significance. 13. HLA and Disease Associations. Index.
Soldano Ferrone received his M.D. and Ph.D. degrees from the University of Milan, Italy. Currently, he is a Professor in the Department of Pathology, College of Physicians and Surgeons, Columbia University in New York. Prior to his present position, Dr. Ferrone was an Associate Member of Scripps Clinic and Research Foundation, La Jolla, Calif., and Adjunct Professor of Pathology at the University of California at San Diego., Bjarte G. Solheim received his M.D. degree in 1965 from the University of Bergen, Norway, and his Ph.D. degree from the University of Oslo, Norway, in 1972. Currently, he is head of the Blood Typing Laboratory, Rikshospitalet, The National Hospital, in Oslo, Norway.