HPLC: Practical and Industrial Applications, Second Edition, 2nd Edition (Hardback) book cover

HPLC

Practical and Industrial Applications, Second Edition, 2nd Edition

Edited by Joel K. Swadesh

CRC Press

480 pages

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pub: 2000-12-21
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Description

Product specifications, regulatory constraints, and tight production schedules impose considerable pressures on separation scientists in industry. The first edition of HPLC: Practical and Industrial Applications helped eliminate the need for extensive library or laboratory research when confronting a problem, an unfamiliar technique, or work in a new area. Its plain language, comprehensive coverage of separation topics, and practical organization made it an accessible and convenient reference manual for anyone working in or just entering the field.

Since its publication in 1997, however, much has changed. The areas of mass spectroscopy, electrophoretic separations, and ultra-micro separations have blossomed, focus on quality control has intensified, and the literature has grown significantly. The Second Edition incorporates all of these changes and more. It is now fully current, with chapter supplements that include updated references and discussions of techniques.

This book examines analytical HPLC as it is actually used in industry. Whether you are just entering industry, switching from one industry to another, or simply enjoy understanding how things are made, HPLC: Practical and Industrial Applications will help you solve problems and get up to speed in new areas quickly, comfortably, and with a genuine sense of mastery.

Table of Contents

INTRODUCTION, Jeffrey R. Larson, James E. Tingstad, and Joel K. Swadesh

Overview

Pumps

Columns

Chromatographic modes

Detectors

Chromatographic theory

Laboratory operations

The role of laboratory management

AUTOMATED SAMPLING IN THE PROCESS ENVIRONMENT, Jeffrey R. Larson

Issues in process HPLC

Case histories

Future directions for on-line HPLC process monitors

Summary

CHROMATOGRAPHY IN PROCESS DEVELOPMENT, Patricia Puma

Scope of process chromatography

Special hardware requirements

Validation considerations

Case history

Summary

REVERSED PHASE HPLC, Rekha D. Shah and Cynthia A. Maryanoff

Introduction

Chromatographic practice in RPLC

Selected applications

Case history: optimizing a chemical synthesis by RPLC

Conclusions

ION EXCHANGE CHROMATOGRAPHY, Joel K. Swadesh

Introduction

Ion exchange stationary phases

Electrochemical detectors

Applications

Case history: isolation and partial characterization of hementin, a compound of biopharmaceutical interest

Acknowledgments

GEL PERMEATION CHROMATOGRAPHY, Rajesh G. Beri, Laurel S. Hacche, and Carl F. Martin

Introduction

Detectors for gel permeation chromatography

Application of aqueous size-exclusion chromatography352 to carbohydrates: a case history

HIGH PERFORMANCE CAPILLARY ELECTROPHORESIS: AN OVERVIEW, Kálmán Benedik and András Guttman

Introduction

Fundamentals

Instrument

Separation modes

Applications

Conclusions

Index

About the Series

Analytical Chemistry

Learn more…

Subject Categories

BISAC Subject Codes/Headings:
SCI013010
SCIENCE / Chemistry / Analytic
SCI013060
SCIENCE / Chemistry / Industrial & Technical