2nd Edition

HPLC
Practical and Industrial Applications, Second Edition




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ISBN 9780849300035
Published December 21, 2000 by CRC Press
480 Pages

USD $195.00

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Book Description

Product specifications, regulatory constraints, and tight production schedules impose considerable pressures on separation scientists in industry. The first edition of HPLC: Practical and Industrial Applications helped eliminate the need for extensive library or laboratory research when confronting a problem, an unfamiliar technique, or work in a new area. Its plain language, comprehensive coverage of separation topics, and practical organization made it an accessible and convenient reference manual for anyone working in or just entering the field.

Since its publication in 1997, however, much has changed. The areas of mass spectroscopy, electrophoretic separations, and ultra-micro separations have blossomed, focus on quality control has intensified, and the literature has grown significantly. The Second Edition incorporates all of these changes and more. It is now fully current, with chapter supplements that include updated references and discussions of techniques.

This book examines analytical HPLC as it is actually used in industry. Whether you are just entering industry, switching from one industry to another, or simply enjoy understanding how things are made, HPLC: Practical and Industrial Applications will help you solve problems and get up to speed in new areas quickly, comfortably, and with a genuine sense of mastery.

Table of Contents

INTRODUCTION, Jeffrey R. Larson, James E. Tingstad, and Joel K. Swadesh
Overview
Pumps
Columns
Chromatographic modes
Detectors
Chromatographic theory
Laboratory operations
The role of laboratory management
AUTOMATED SAMPLING IN THE PROCESS ENVIRONMENT, Jeffrey R. Larson
Issues in process HPLC
Case histories
Future directions for on-line HPLC process monitors
Summary
CHROMATOGRAPHY IN PROCESS DEVELOPMENT, Patricia Puma
Scope of process chromatography
Special hardware requirements
Validation considerations
Case history
Summary
REVERSED PHASE HPLC, Rekha D. Shah and Cynthia A. Maryanoff
Introduction
Chromatographic practice in RPLC
Selected applications
Case history: optimizing a chemical synthesis by RPLC
Conclusions
ION EXCHANGE CHROMATOGRAPHY, Joel K. Swadesh
Introduction
Ion exchange stationary phases
Electrochemical detectors
Applications
Case history: isolation and partial characterization of hementin, a compound of biopharmaceutical interest
Acknowledgments
GEL PERMEATION CHROMATOGRAPHY, Rajesh G. Beri, Laurel S. Hacche, and Carl F. Martin
Introduction
Detectors for gel permeation chromatography
Application of aqueous size-exclusion chromatography352 to carbohydrates: a case history
HIGH PERFORMANCE CAPILLARY ELECTROPHORESIS: AN OVERVIEW, Kálmán Benedik and András Guttman
Introduction
Fundamentals
Instrument
Separation modes
Applications
Conclusions
Index

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Editor(s)

Biography

Swadesh\, Joel K.