Handbook of Affinity Chromatography: 2nd Edition (Hardback) book cover

Handbook of Affinity Chromatography

2nd Edition

Edited by David S. Hage, Jack Cazes

CRC Press

968 pages | 327 B/W Illus.

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pub: 2005-07-19
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This essential handbook guides investigators in the theory, applications, and practical use of affinity chromatography in a variety of fields including biotechnology, biochemistry, molecular biology, analytical chemistry, proteomics, pharmaceutical science, environmental analysis, and clinical chemistry.

The Handbook of Affinity Chromatography reflects upon important factors to consider in the development of affinity methods, such as the choice of support material, immobilization methods, and application or elution conditions. It reviews common affinity methods and explores the latest preparative, analytical, and biophysical applications, including the use of affinity chromatography with other separation techniques and analytical systems. This basis seamlessly supports the discussion of recent developments in techniques including the use of affinity ligands in capillary electrophoresis, mass spectrometry, microanalytical systems, and optical biosensors. New chapters feature expanded discussions on molecularly imprinted polymers and biomimetic ligands, chromatographic immunoassays, affinity-based immunoassays, affinity-based chiral stationary phases, and affinity ligands in multidimensional systems.

Engaging the collaboration of 48 scientists and students from 23 laboratories and organizations to present the latest information on affinity methods, the Handbook of Affinity Chromatography illustrates a wide range of applications and theory for scientists, students, and laboratory workers throughout the fields of chemistry and biology.


“The topics are well illustrated and range from fields of biochemistry, molecular biology and biotechnology to analytical chemistry, pharmaceutical and environmental science. This handbook can be an excellent source of information not only to the students but also to the persons involved in research and academia.”

Carbohydrate Polymers

Table of Contents


An Introduction to Affinity Chromatography; D.S. Hage and P.F. Ruhn

Support Materials for Affinity Chromatography; P. Gustavsson and P. Larsson

Immobilization Methods for Affinity Chromatography; H.S. Kim and D.S. Hage

Application and Elution in Affinity Chromatography; D.S. Hage, H. Xuan, and M.A. Nelson


Bioaffinity Chromatography; D.S. Hage, M. Bian, R. Burks, E. Karle, C. Ohnmacht, and C. Wa

Immunoaffinity Chromatography; D.S. Hage and T.M. Phillips

DNA Affinity Chromatography; R.A. Moxley, S. Oak, H. Gadgil, and H.W. Jarrett

Boronate Affinity Chromatography; X. Liu and W.H. Scouten

Dye-Ligand and Biomimetic Affinity Chromatography; N.E. Labrou, K. Mazitsos, and Y.D. Clonis

Immobilized Metal-Ion Affinity Chromatography; D. Todorova and M.A. Vijayalakshmi


General Considerations in Preparative Affinity Chromatography; A. Subramanian

Affinity Chromatography of Enzymes; F. Friedberg and A.R. Rhoads

Isolation of Recombinant Proteins by Affinity Chromatography; A. Subramanian

Affinity Chromatography in Antibody and Antigen Purification; T.M. Phillips

Affinity Chromatography of Regulatory and Signal-Transducing Proteins; A.R. Rhoads and F. Friedberg

Receptor-Affinity Chromatography; P. Bailon, M. Nachman-Clewner, C. L. Spence, and G.K. Ehrlich


Affinity Methods in Clinical and Pharmaceutical Analysis; Carrie A.C. Wolfe, W. Clarke and D.S. Hage

Affinity Chromatography in Biotechnology; N. Jordan and I. Krull

Environmental Analysis by Affinity Chromatography; M.A. Nelson and D.S. Hage

Affinity Chromatography in Molecular Biology; L.A. Jurado, S.Oak, Himanshu Gadgil, R.A. Moxley, and H.W. Jarrett

Affinity-Based Chiral Stationary Phases; S. Patel, I.W. Wainer and W.J. Lough


Quantitative Affinity Chromatography: Practical Aspects; D.S. Hage and J. Chen

Quantitative Affinity Chromatography: Recent Theoretical Developments; D.J. Winzor

Chromatographic Studies of Molecular Recognition and Solute Binding to Enzymes and Plasma Proteins; S. Patel, I.W. Wainer and W.J. Lough

Affinity-Based Optical Biosensors; S.D. Long and D.G. Myszka


Affinity Ligands in Capillary Electrophoresis; Niels H. H. Heegaard and C. Schou

Affinity Mass Spectrometry; C.J. Briscoe, W. Clarke and D.S. Hage

Microanalytical Methods Based on Affinity Chromatography; T.M. Phillips

Chromatographic Immunoassays; A.C. Moser and D.S. Hage

Molecularly Imprinted Polymers: Artificial Receptors for Affinity Separations; K. Haupt

About the Series

Chromatographic Science Series

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Subject Categories

BISAC Subject Codes/Headings:
SCIENCE / Chemistry / Industrial & Technical
SCIENCE / Research & Methodology