Handbook of Biomedical Optics: 1st Edition (Hardback) book cover

Handbook of Biomedical Optics

1st Edition

Edited by David A. Boas, Constantinos Pitris, Nimmi Ramanujam

CRC Press

831 pages | 44 Color Illus. | 486 B/W Illus.

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pub: 2011-06-14
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Biomedical optics holds tremendous promise to deliver effective, safe, non- or minimally invasive diagnostics and targeted, customizable therapeutics. Handbook of Biomedical Optics provides an in-depth treatment of the field, including coverage of applications for biomedical research, diagnosis, and therapy. It introduces the theory and fundamentals of each subject, ensuring accessibility to a wide multidisciplinary readership. It also offers a view of the state of the art and discusses advantages and disadvantages of various techniques.

Organized into six sections, this handbook:

  • Contains introductory material on optics and the optical properties of tissue
  • Describes the various forms of spectroscopy and its applications in medicine and biology, including methods that exploit intrinsic absorption and scattering contrast; dynamic contrast; and fluorescence and Raman contrast mechanisms
  • Provides extensive coverage of tomography from the microscopic (optical coherence tomography) to the macroscopic (diffuse optical tomography) to photoacoustic tomography
  • Discusses cutting-edge translations to biomedical applications in both basic sciences and clinical studies
  • Details molecular imaging and molecular probe development
  • Highlights the use of light in disease and injury treatment

The breadth and depth of multidisciplinary knowledge in biomedical optics has been expanding continuously and exponentially, thus underscoring the lack of a single source to serve as a reference and teaching tool for scientists in related fields. Handbook of Biomedical Optics addresses this need, offering the most complete up-to-date overview of the field for researchers and students alike.


"… a great resource for scientists and graduate students in biomedical engineering and optics. In addition to providing background information on classical optics, irradiation guidelines and tissue optical properties, this book offers a thorough treatment of tomographic and microscopic imaging technologies."

Optics & Photonics News, January 2013

"This handbook is a successful attempt to provide as much as possible information to a number of physicists, R&D engineers, clinicians and scientists who would like to apply contemporary concepts and techniques to solving biomedical diagnostic and therapeutic challenges. It often covers areas that are not conventionally addressed, and is generally thought-provoking and brain-stimulating … . The book can serve as an excellent tutorial for students and young investigators finding their way through the intense and exciting field of biomedical optics. It is a relevant reference for scientists and also a good teaching tool. … a good balance between introductory knowledge and high-level expertise, without burdening the reader with excessive data. What makes it really unique is its scope. This is a handbook of thirty-five chapters, covering an enormous breadth of material… . An additional value is that the book is truly state of the art."

—Boris Gramatikov, Johns Hopkins University School of Medicine, Biomedical Engineering Online, February 2012

"Handbook of Biomedical Optics is a useful addition as an overall source to help educate the new and present generation of graduate students, researchers, and medical professionals. … highly recommend it. It provides an in-depth review and overall treatment covering most areas of biomedicine from the ground-level foundations of optics to background on photon transport in tissues, theory, and experiments. … Handbook of Biomedical Optics is a major contribution to the biomedical optics field and will serve as a great resource."

Journal of Biomedical Optics, December 2011

Table of Contents


David A. Boas, Constantinos Pitris, Nimmi Ramanujam

I. Background

Geometrical Optics

Ting-Chung Poon

Diffraction Optics

Colin Sheppard

Optics: Basic Physics

Raghuveer Parthasarathy

Light Sources, Detectors, and Irradiation Guidelines

Carlo Amadeo Alonzo, Malte C. Gather, Jeon Woong Kang, Giuliano Scarcelli, Seok-Hyun Yun

Tissue Optical Properties

Alexey N. Bashkatov, Elina A. Genina, Valery V. Tuchin

II. Spectroscopy and Spectral Imaging

Reflectance Spectroscopy

Sasha McGee, Jelena Mirkovic, Michael Feld

Multi/Hyper-Spectral Imaging

Costas Balas, Christos Pappas, George Epitropou

Light Scattering Spectroscopy

Le Qiu, Irving Itzkan, Lev T. Perelman

Broadband Diffuse Optical Spectroscopic Imaging

Bruce J. Tromberg, Albert E. Cerussi, So-Hyun Chung, Wendy Tanamai, Amanda Durkin

Near Infrared Diffuse Correlation Spectroscopy for Assessment of Tissue Blood Flow

Guoqiang Yu, Turgut Durduran, Chao Zhou, Ran Cheng, Arjun G. Yodh

Fluorescence Spectroscopy

Darren Roblyer, Richard A. Schwarz, Rebecca Richards-Kortum

Raman, SERS and FTIR Spectroscopy

Andrew J. Berger

III. Tomographic Imaging

Optical Coherence Tomography: Introduction and Theory

Yu Chen, Evgenia Bousie, Constantinos Pitris, James G. Fujimoto

Functional Optical Coherence Tomography in Preclinical Models

Melissa C. Skala, Yuankai K. Tao, Anjul M. Davis, Joseph A. Izatt

Optical Coherence Tomography: Clinical Applications

Brian D. Goldberg, Melissa J. Suter, Guillermo J. Tearney, Brett E. Bouma

Forward Models of Light Transport in Biological Tissue

Andreas H. Hielscher, Hyun Keol Kim, Alexander K. Klose

Inverse Models of Light Transport

Simon Arridge, Martin Schweiger, John C. Schotland

Laminar Optical Tomography

Sean A. Burgess, Elizabeth M. C. Hillman

Diffuse Optical Tomography using Continuous Wave and Frequency Domain Imaging Systems

Subhadra Srinivasan, Scott C. Davis, Colin M. Carpenter

Diffuse Optical Tomography: Time Domain

Juliette Selb, Adam Gibson

Photoacoustic Tomography and Ultrasound-Modulated Optical Tomography

Changhui Li, Chulhong Kim, Lihong V. Wang

Optical and Photoacoustic Molecular Tomography of Small Animals

Vasilis Ntziachristos

IV. Microscopic Imaging

Assesing Microscopic Structural Features Using Fourier-Domain Low Coherence Interferometry

Robert N. Graf, Francisco E. Robles, Adam Wax

Phase Imaging Microscopy: Beyond Darkfield, Phase and Differential Interference Contrast Microscopy

Chrysanthe Preza, Sharon V. King, Nicoleta M. Dragomir, Carol J. Cogswell

Confocal Microscopy

William C. Warger II, Charles A. DiMarzio, Milind Rajadhyaksha

Fluorescence Microscopy with Structured Excitation Illumination

Alexander Brunner, Gerrit Best, Roman Amberger, Paul Lemmer, Thomas Ach, Stefan Dithmar, Rainer Heintzmann, Christoph Cremer

Nonlinear Optical Microscopy for Biology and Medicine

Daekeun Kim, Heejin Choi, Jae Won Cha, Peter T. C. So

Fluorescence Lifetime Imaging Microscopy, Endoscopy and Tomography

James McGinty, Clifford Talbot, Dylan Owen, David Grant, Sunil Kumar, Neil Galletly, Bebhinn Treanor, Gordon Kennedy, Peter M. P. Lanigan, Ian Munro, Daniel S. Elson, Anthony Magee, Dan Davis, Gordon Stamp, Mark Neil, Christopher Dunsby, Paul W. M. French

Application of Digital Holographic Microscopy in Biomedicine

Christian Depeursinge, Pierre Marquet, Nicolas Pavillon

Polarized Light Imaging of Biological Tissues

Steven L. Jacques

V. Molecular Probe Development

Molecular Reporter Systems for Optical Imaging

Walter J. Akers, Samuel Achilefu

Nanoparticles for Targeted Therapeutics and Diagnostics

Timothy Larson, Kort Travis, Pratixa Joshi, Konstantin Sokolov

Plasmonic Nanoprobes for Biomolecular Diagnostics of DNA Targets

Tuan Vo-Dinh, Hsin-Neng Wang

VI. Phototherapy

Photodynamic Therapy

Jarod C. Finlay, Keith Cengel, Theresa M. Busch, Timothy C. Zhu

Low Level Laser and Light Therapy

Ying-Ying Huang, Aaron C-H Chen, Michael R. Hamblin

About the Editors

Dr. David A. Boas is an Associate Professor at the Harvard Medical School and Associate Physicist at Massachusetts General Hospital in Boston, Massachusetts. He received his Bachelors Degree in Physics from Rensselaer Polytechnic Institute, Troy NY in 1991 and his Doctorate from the University of Pennsylvania, Philadelphia, PA, also in Physics. His research interests include the following: photon migration in highly scattering media with emphasis on diffuse optical tomography, clinical applications of diffuse optical tomography in brain and breast radiology and fundamental studies of brain function and stroke using diffuse optical tomography and optical microscopy. Dr. Boas has been an Associate Editor of Optics Express and Guest Editor of Medical Physics and Journal of Biomedical Optics. He is a member of SPIE and the Optical Society of America (OSA), and has served as Conference Program Chair for various OSA topical meetings.

Dr. Constantinos Pitris is an Assistant Professor in the faculty of Electrical and Computer Engineering at the University of Cyprus. He completed his studies at the University of Texas at Austin (BS Honors in Electrical Engineering, 1993, MS in Electrical Engineering, 1995), Massachusetts Institute of Technology (Ph.D. in Electrical and Medical Engineering, 2000), and Harvard Medical School (MO Magna Cum Laude in Medicine, 2002). He has worked as a research assistant at the University of Texas and Massachusetts Institute of Technology and as a postdoctoral associate at the Wellman Laboratories of Photomedicine of the Massachusetts General Hospital and Harvard Medical School. His main research interests cover the areas of optics and biomedical imaging. The goal of this research is the introduction of new technologies in clinical applications for the improvement of diagnostic and therapeutic options. He is an active member of the OSA and a reviewer for Optics Letters, Applied Optics and Biomedical Optics.

Dr. Nimmi Ramanujam is an Associate Professor of Biomedical Engineering at Duke University. Dr. Ramanujam earned her Ph.D. in Biomedical Engineering from the University of Texas, Austin in 1995 and trained as an NIH postdoctoral fellow at the University of Pennsylvania from 1996-2000. Prior to her tenure at Duke, she was an assistant professor in the Department of Biomedical Engineering at the University of Wisconsin, Madison from 2000-2005. Dr. Ramanujam's interests in the field of biophotonics are centered on research and technology development for applications to cancer. She is developing novel quantitative optical sensing and imaging tools for translational applications in cancer research. She has been leading a multidisciplinary effort to translate these technologies into pre-clinical models and cancer patients. Dr. Ramanujam is a fellow of the OSA and was invited to be a panel member for the Department of Defense (DOD) Breast Cancer Research Program (BCRP) Integration Panel. She has received several awards for her work in cancer research and technology development, including Era of Hope Scholar awards from the DOD and a Global Indus Technovator award from MIT.

Subject Categories

BISAC Subject Codes/Headings:
MEDICAL / Biotechnology
SCIENCE / Optics