Still widely used as gene markers, isozymes detected by zymogram techniques have proven valuable in a range of other biological applications over the last few years. Along with these new applications, many new techniques have also emerged. Yet more than eight years since the Handbook of Detection of Enzymes on Electrophoretic Gels was first published, it remains the only book completely devoted to zymogram methods. The time has come to bring its contents up to date.
New in the Second Edition:
The second edition of this bestselling handbook makes significant additions to the set of reliable gene markers suitable for electrophoretic analysis. It also strengthens the value of enzyme electrophoresis as a powerful tool proven successful in solving a variety of problems encountered across many biological areas, including the post-genome biology.
Table of Contents
INTRODUCTION. Enzyme Electrophoresis. Zymogram Techniques. Genetic Bases of Multiple Molecular Forms of Enzymes Developed on Zymograms. Applications. GENERAL PRINCIPLES OF ENZYME DETECTION ON ELECTROPHORETIC GELS. Chromogenic Reactions. Fluorogenic Reactions. Autoradiography. Bioautography. Two-Dimensional Gel Spectroscopy. Immunoblotting. METHODS OF DETECTION OF SPECIFIC ENZYMES. The Structure of Enzyme Sheets. General Considerations, Comments, and Recommendations. Enzyme Sheets. APPENDICES.
Gennady P. Manchenko, Ph.D., is senior researcher at the Laboratory of Genetics at the Institute of Marine Biology of the Russian Academy of Sciences at Vladivostok.