Handbook of Detection of Enzymes on Electrophoretic Gels: 2nd Edition (Hardback) book cover

Handbook of Detection of Enzymes on Electrophoretic Gels

2nd Edition

By Gennady P. Manchenko

CRC Press

568 pages | 649 B/W Illus.

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Hardback: 9780849312571
pub: 2002-12-26
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Description

Still widely used as gene markers, isozymes detected by zymogram techniques have proven valuable in a range of other biological applications over the last few years. Along with these new applications, many new techniques have also emerged. Yet more than eight years since the Handbook of Detection of Enzymes on Electrophoretic Gels was first published, it remains the only book completely devoted to zymogram methods. The time has come to bring its contents up to date.

New in the Second Edition:

  • An overview of new applications of enzyme electrophoresis and zymogram techniques

  • Zymogram techniques for approximately 100 enzymes not included in the first edition, bringing the total to more than 900 methods for detecting more than 400 different enzymes

  • Information on subunit structure included within the enzyme sheets to facilitate interpretation of isozyme patterns detected on zymograms

  • An appendix containing information on the buffer systems most commonly used for enzyme electrophoresis in starch, cellulose acetate, and polyacrylamide gels.

    The second edition of this bestselling handbook makes significant additions to the set of reliable gene markers suitable for electrophoretic analysis. It also strengthens the value of enzyme electrophoresis as a powerful tool proven successful in solving a variety of problems encountered across many biological areas, including the post-genome biology.

  • Table of Contents

    INTRODUCTION

    Enzyme Electrophoresis

    Zymogram Techniques

    Genetic Bases of Multiple Molecular Forms of Enzymes Developed on Zymograms

    Applications

    GENERAL PRINCIPLES OF ENZYME DETECTION ON ELECTROPHORETIC GELS

    Chromogenic Reactions

    Fluorogenic Reactions

    Autoradiography

    Bioautography

    Two-Dimensional Gel Spectroscopy

    Immunoblotting

    METHODS OF DETECTION OF SPECIFIC ENZYMES

    The Structure of Enzyme Sheets

    General Considerations, Comments, and Recommendations

    Enzyme Sheets

    APPENDICES.

    About the Author

    Gennady P. Manchenko, Ph.D., is senior researcher at the Laboratory of Genetics at the Institute of Marine Biology of the Russian Academy of Sciences at Vladivostok.

    Subject Categories

    BISAC Subject Codes/Headings:
    MED008000
    MEDICAL / Biochemistry
    SCI029000
    SCIENCE / Life Sciences / Genetics & Genomics
    SCI049000
    SCIENCE / Life Sciences / Biology / Molecular Biology
    SCI086000
    SCIENCE / Life Sciences / General