This book provides comprehensive, detailed, and step-by-step descriptions of materials and methods currently used in laboratory procedures within the infertility clinic. It addresses the numerous highly sophisticated procedures resulting from research in the area of laboratory diagnosis and treatment of infertility.
The Infertility Evaluation. The Endometrial Biopsy. The Semen Analysis. Critical Evaluation of Sperm Morphology; Comparison with In Vitro Fertilization. Biochemical Analysis of Seminal Plasma and Spermatozoa. Objective Analysis of Sperm Motility and Kinematics. Methodology of the Optimized Sperm Penetration Assay. Sperm-Cervical Mucus Interaction. Detection of Agglutinating and Immobolizing Antisperm Antibodies. Detection of Antisperm Antibodies Using Immunobeads. Sperm Washing and Concentration Techniques. The Hemi-Zona Assay (HZA): Assessment of Fertilizing Potential by Means of Human Sperm Binding to Human Zona Pellucida. Cryopreservation of Human Semen. Microbiological Examination of the Infertile Couple. Statistical Methods for Serum Hormone Assays. Organization of the In Vitro Fertilization and Embryo Transfer Laboratory. Mouse Preembryo Culture as an Evaluation for Human Preembryo Requirements. Oocyte and Embryo Cryopreservation Techniques and Results. The Morphological Assessment of Human Oocytes and Early Concepti. Index.