HPLC is the principal separation technique for identification of the pesticides in environmental samples and for quantitative analysis of analytes. At each stage of the HPLC procedure, the chromatographer should possess both the practical and theoretical skills required to perform HPLC experiments correctly and to obtain reliable, repeatable, and reproducible results.
Developed to serve as a detailed practical guide, High Performance Liquid Chromatography in Pesticide Residue Analysis is a comprehensive source of information and training on state-of-the-art pesticide residue methods performed with the aid of HPLC. The book presents the pros and cons of HPLC as a flexible and versatile separation and analysis tool with multiple purposes and advantages in investigations of pesticides for food and plant drugs standardization, promotion of health, protection of new herbal medicines, and more.
Table of Contents
PRACTICAL GUIDE TO HPLC METHODS OF PESTICIDE RESIDUE ANALYSIS. Overview of the Field of Chromatographic Methods of Pesticide Residue Analysis and Organization of the Book. Pesticide Classification and Properties. Method Development of Chromatography: Retention–Eluent Composition Relationships and Application to Analysis of Pesticides. Choice of the Mode of Chromatographic Method for Analysis of Pesticides on the Basis of the Properties of Analytes. Choice of the Mode of Sample Preparation in the Analysis of Pesticides on the Basis of the Properties of the Matrix. Selection of the Mode of Stationary Phases and Columns for Analysis of Pesticides. Selection of the Type of Mobile Phases for Analysis of Nonionic Analytes: Reversed- and Normal-Phase HPLC. Selection of the Mobile Phases for Analysis of Ionic Analytes: Reversed- Phase, Ion-Pair, Ion-Exchange, Ion-Exclusion HPLC. Optimization of Normal-Phase and Reversed-Phase Systems for Analysis of Pesticides: Choice of the Mode of Elution–Isocratic and Gradient Elution. KINETIC STUDY OF PESTICIDES. Kinetics Study of Pesticides in the Environment: Application of HPLC to Kinetic Effects of Pesticide Analysis. Phototransformation of Pesticides in the Environment. APPLICATIONS OF HPLC AND UPLC TO SEPARATION AND ANALYSIS OF PESTICIDES FROM VARIOUS CLASSES. Sample Preparation for Determination of Pesticides by High-Performance Liquid Chromatography and Liquid Chromatography–Tandem Mass Spectrometry. Quantitative Analysis and Method Validation. Analysis of Pesticides by HPLC–UV, HPLC–DAD (HPLC–PDA), and Other Detection Methods. High-Performance Liquid Chromatography–Mass Spectrometry as a Method of Identification and Quantification of Pesticides. Multidimensional Liquid Chromatography Applied to the Analysis of Pesticides. Chiral Separation of Some Classes of Pesticides by High-Performance Liquid Chromatography Method. Application in Pesticide Analysis: Liquid Chromatography—A Review of the State of Science for Biomarker Discovery and Identification. Ultra-Performance Liquid Chromatography Applied to Analysis of Pesticides. High-Performance Liquid Chromatography versus Other Modern Analytical Methods for Determination of Pesticides.
Tomasz Tuzimski, Joseph Sherma