1st Edition

High Throughput Screening The Discovery of Bioactive Substances

By John P. Devlin Copyright 1997

    Furnishing the latest interdisciplinary information on the most important and frequently the only investigational system available for discovery programs that address the effects of small molecules on newly discovered enzyme and receptor targets emanating from molecular biology, this timely resource facilitates the transition from classical to high throughput screening (HTS) systems and provides a solid foundation for the implementation and development of HTS in bio-based industries and associated academic environments.

    Natural Products as a Discovery Resource, edited by John P. Devlin

    Chemical Diversity and Genetic Equity: Synthetic and Naturally Derived Compounds, John P. Devlin
    Microcollection of Plants for Biochemical Profiling, John P. Devlin
    Enzymes and Microbes as a Source of Chemical Diversity, Saul L. Neidleman
    The Marine Environment as a Discovery Resource, Gregg R. Dietzman

    Compound Sourcing: Chemically Generated Screening Libraries, edited by Michael R. Pavia

    Introduction, Michael R. Pavia
    Rapidly Expanding Molecular Diversity: Libraries from Libraries, John S. Kiely, Barbara Dörner, John M. Ostresh, Colette Dooley, and Richard A. Houghten
    Synthesis of Encoded Small Molecule Combinatorial Libraries via ECLiPS, Jack J. Baldwin and Ian Henderson
    Parallel Organic Synthesis Using Parke-Davis' Diversomer® Method, Sheila Hobbs DeWitt and Anthony W. Czarnik
    Rapid Discovery and Optimization of Biologically Active Small Molecules Using Automated Synthesis Methods, Adnan M. M. Mjalli and Barry E. Toyonaga
    CMT: A Solution Phase Combinatorial Chemistry Approach: Synthesis and Yield Prediction Phenazines, Ferenc Darvas and László Kovács
    Design of a Diverse Screening Library, David E. Patterson, Allan M. Ferguson, Richard D. Cramer, Cheryl D. Garr, Ted L. Underiner, and John R. Peterson
    Automating Combinatorial Chemistry: Challenges and Pitfalls, Jill H. Hardin and Frank R. Smietana
    Combi-chem High Throughput Screening for Leads Optimization, Richard M. Kris, Patti Willson, Serene Josiah, Marianne Wildgoose, Helen Yeoman, Viktor Krchnak, Stephen Baum, and Stephen Felder

    Assay Technologies and Detection Methods, edited by Alfred J. Kolb

    Introduction, Alfred J. Koin
    Bioassay Design and Implementation, Robert W. Wallace and Mark E. Goldman
    Scintillation Proximity Assays, Martino Picardo and Kelvin T. Hughes
    FlashPlate™ Technology, Beverly A. Brown, Margaret Cain, Jeff Broadbent, Sharon Tompkins, Georgette Henrich, Rich Joseph, Sally Casto, Harry Harney, Rick Greene, Ron Delmondo, and Simon Ng
    Assays for Small Molecule Agonists and Antagonists of the Neurotrophin Receptors, Maureen H. Beresini, Michael D. Sadick, Amy L. Galloway, Randy Yen, Sherry H. Yeh, Andy C. Chang, David L. Shelton, and Wai Lee T. Wong
    A Homogeneous, Time-Resolved Fluorescence Method for Drug Discovery, Alfred J. Kolb, Jocelyn W. Burke, and Gerard Mathis
    Time-Resolved Fluorometry: Advantages and Potentials, Ilkka Hemmilä
    Adaptation of Time-Resolved Fluorescence to Homogeneous Screening Formats, Janet M. Kolb, Greg Yamanaka, and Susan P. Manly
    Fluorescence Polarization, J. Richard Sportsman, Sandra K. Lee, Hara Dilley, and Robert Bukar
    Reporter Gene Assay Applications, Winfried Scheirer
    Development of a Gene Expression-Based Screen Using Quantitative Polymerase Chain Reaction, D. K. H. Lee, E. T. Wilkinson, S. Cheifetz, and S. A. De Grandis
    High-Performance Microphysiometry in Drug Discovery, M. Liisa Alajoki, Gregory T. Baxter, William R. Bemiss, David Blau, Luc J. Bousse, Samuel D. H. Chan, Timothy D. Dawes, Karen M. Hahnenberger, James M. Hamilton, Philip Lam, Richard J. McReynolds, Douglas N. Modlin, John C. Owicki, J. Wallace Parce, Dana Redington, Knute Stevenson, H. Garrett Wada, and Jana Williams
    Bioanalytical Applications of BIAcore, an Optical Biosensor, Francois Legay, Patrick Albientz, and Rüdiger Ridder

    Automation and Robotics, edited by Alfred J. Kolb

    Introduction, Alfred J. Kolb
    Management and Service Issues of a Centralized Robotic HTS Core, John Babiak
    Flexible Use of People and Machines, Mark Beggs and John S. Major
    Bar-Code Technology and a Centralized Database: Key Components in a Radioligand Binding Program, Elizabeth A. Kunysz, Melissa Lukes, and Douglas W. Bonhaus
    Factors for the Successful Integration of Assays, Equipment, Robotics, and Software, Dejan Bojanic, Wilma W. Keighley, Mike J. Russell, and Terry P. Wood
    Accelerating the Discovery Process with Automation and Robotics: A Sure Bet or a Risky Venture?, Richard K. Brown and Andree Proulx
    Perspectives on Scheduling, Alain Donzel, Jorge Carmona, and L. Andrew Corkan

    Data Retrieval, Handling, and Integration, edited by John P. Devlin

    Introduction, John P. Devlin
    Database Systems, Barr E. Bauer
    Systems Integration, Chip Allee
    Data Management and Tracking for Natural Product Programs, Gregg R. Dietzman

    Laboratory Design and Management, edited by Matthew A. Sills

    Introduction, Matthew A. Sills
    Planning and Implementing an HTS Program, Matthew A. Sills and Mark Crawford
    Establishing an HTS Program in a Start-up Biotechnology Company, Michael D. Boisclair, James Schultz, and Dan Maloney


    John P. Devlin

    …a valuable reference.
    "-Journal of Pharmacy and Pharmacology

    "…a well-referenced, highly professional, comprehensive, and in-depth treatment of a relatively complex approach to the discovery of bioactive substances….
    …a recommended book for most private libraries of both academic and industrial pharmaceutical and agricultural chemists and biologists and a must acquisition for applicable and industrial libraries.
    "-Journal of Medicinal Chemistry

    "…an excellent compendium…covering the breath of current HTS. It will serve as a standard text in the field.
    "-Journal of Natural Products

    This recommendable book should be of great value for the entry in high throughput screening systems in industry and research. It gives a very useful and clear description of the development of HTS systems from finding resources and generating libraries to assay and detection methods and finally automation, data handling and laboratory design.
    - European Journal of Pharmaceutics and Biopharmaceutics, 2000