1st Edition

Histochemical and Cytochemical Methods of Visualization

Edited By Jean-Marie Exbrayat Copyright 2013
    367 Pages 33 Color & 193 B/W Illustrations
    by CRC Press

    367 Pages 33 Color & 193 B/W Illustrations
    by CRC Press

    Histochemistry and cytochemistry are important fields for studying the inner workings of cells and tissues of the body. While visualization techniques have been in use for many years, new methods of detection developed recently help researchers and practitioners better understand cell activity. Histochemical and Cytochemical Methods of Visualization describes the essential techniques that can be used for histochemical investigations in both light and transmission electron microscopy.

    The book begins by discussing techniques in light microscopy. It reviews classical methods of visualization, histochemical and histoenzymatic methods, and methods used to visualize cell proliferation and apoptosis. Next, the book examines the cytochemical methods used in electron microscopy with traditional techniques, as well as more specialized methods. The final section provides an overview of image analysis and describes how image processing methods can be used to extract vital information. A 16-page insert supplies color illustrations to enhance the text.

    Techniques will continue to adapt to the latest technological innovations, allowing more and more precise quantification of images. These developments are essential to the biological as well as the medical sciences. This manual is a critical resource for novice and experienced researchers, technicians, and students who need to visualize what happens in the cell, the molecules expressed, the main enzymatic activities, and the repercussions of the molecular activities upon the structure of the cells in the body.


    Histological and Histochemical Methods with Light Microscopy
    Classical Methods of Visualization; Jean-Marie Exbrayat
    Histochemical Methods; Jean-Marie Exbrayat
    Enzyme Histochemistry Methods; Jeanne Estabel
    Visualization of Cell Proliferation; Françoise Giroud and Marie-Paule Montmasson
    Visualization of Apoptosis; Claire Brun and Elara Moudilou
    In Situ
    High-Speed Tissue Proteomic; Françoise Giroud and Marie-Paule Montmasson
    Preparation of Products for Histology and Histochemistry; Jean-Marie Exbrayat
    Cytological Methods in Electron Microscopy
    General Techniques in Electron Microscopy; Jean-Marie Exbrayat
    Cytochemical Techniques; Jean-Marie Exbrayat
    Ultrastructural Cytoenzymology; Chantal de Chastellier
    The Acetylation Method; Marc Thiry and Nicolas Thelen
    Preparation of Products for Electron Microscopy; Jean-Marie Exbrayat
    Image Quantification
    Image Quantification in Histology and Cytology; Yves Tourneur and Léon Espinosa


    Jean-Marie Exbrayat, Ph.D., D.Sc., is a professor at Catholic University of Lyon (France) where he manages the Laboratory of General Biology. He is also directeur d’etudes (professor) at the Ecole Pratique des Hautes Etudes, where he manages the Laboratory of Comparative Reproduction and Development. Dr. Exbrayat earned his M.S. and Ph.D. in 1974 and 1977, respectively, from Montpellier University (France). He earned his D. Sci. in 1986 from Paris 6 University. He teaches animal biology, histology and embryology, and history and philosophy of sciences. He was the head of the School of Histology for 25 years, and dean of the Faculty of Sciences (1997 until 2005). Dr. Exbrayat is a member of several scientific societies. He has published more than 300 papers and 12 books. His current major interests include the variations of genital tracts and endocrine organs during reproductive cycles related to external and internal factors, and embryonic development in both lower and higher vertebrates, as well as the history and philosophy of sciences, especially evolutionary biology.