Histochemical and Cytochemical Methods of Visualization: 1st Edition (Hardback) book cover

Histochemical and Cytochemical Methods of Visualization

1st Edition

Edited by Jean-Marie Exbrayat

CRC Press

367 pages | 33 Color Illus. | 193 B/W Illus.

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Histochemistry and cytochemistry are important fields for studying the inner workings of cells and tissues of the body. While visualization techniques have been in use for many years, new methods of detection developed recently help researchers and practitioners better understand cell activity. Histochemical and Cytochemical Methods of Visualization describes the essential techniques that can be used for histochemical investigations in both light and transmission electron microscopy.

The book begins by discussing techniques in light microscopy. It reviews classical methods of visualization, histochemical and histoenzymatic methods, and methods used to visualize cell proliferation and apoptosis. Next, the book examines the cytochemical methods used in electron microscopy with traditional techniques, as well as more specialized methods. The final section provides an overview of image analysis and describes how image processing methods can be used to extract vital information. A 16-page insert supplies color illustrations to enhance the text.

Techniques will continue to adapt to the latest technological innovations, allowing more and more precise quantification of images. These developments are essential to the biological as well as the medical sciences. This manual is a critical resource for novice and experienced researchers, technicians, and students who need to visualize what happens in the cell, the molecules expressed, the main enzymatic activities, and the repercussions of the molecular activities upon the structure of the cells in the body.


Table of Contents

Histological and Histochemical Methods with Light Microscopy

Classical Methods of Visualization; Jean-Marie Exbrayat

Histochemical Methods; Jean-Marie Exbrayat

Enzyme Histochemistry Methods; Jeanne Estabel

Visualization of Cell Proliferation; Françoise Giroud and Marie-Paule Montmasson

Visualization of Apoptosis; Claire Brun and Elara Moudilou

In Situ High-Speed Tissue Proteomic; Françoise Giroud and Marie-Paule Montmasson

Preparation of Products for Histology and Histochemistry; Jean-Marie Exbrayat

Cytological Methods in Electron Microscopy

General Techniques in Electron Microscopy; Jean-Marie Exbrayat

Cytochemical Techniques; Jean-Marie Exbrayat

Ultrastructural Cytoenzymology; Chantal de Chastellier

The Acetylation Method; Marc Thiry and Nicolas Thelen

Preparation of Products for Electron Microscopy; Jean-Marie Exbrayat

Image Quantification

Image Quantification in Histology and Cytology; Yves Tourneur and Léon Espinosa


About the Editor

Jean-Marie Exbrayat, Ph.D., D.Sc., is a professor at Catholic University of Lyon (France) where he manages the Laboratory of General Biology. He is also directeur d’etudes (professor) at the Ecole Pratique des Hautes Etudes, where he manages the Laboratory of Comparative Reproduction and Development. Dr. Exbrayat earned his M.S. and Ph.D. in 1974 and 1977, respectively, from Montpellier University (France). He earned his D. Sci. in 1986 from Paris 6 University. He teaches animal biology, histology and embryology, and history and philosophy of sciences. He was the head of the School of Histology for 25 years, and dean of the Faculty of Sciences (1997 until 2005). Dr. Exbrayat is a member of several scientific societies. He has published more than 300 papers and 12 books. His current major interests include the variations of genital tracts and endocrine organs during reproductive cycles related to external and internal factors, and embryonic development in both lower and higher vertebrates, as well as the history and philosophy of sciences, especially evolutionary biology.

About the Series

Methods in Visualization

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Subject Categories

BISAC Subject Codes/Headings:
SCIENCE / Life Sciences / Cytology
SCIENCE / Microscopes & Microscopy
SCIENCE / Life Sciences / Biology / Molecular Biology
SCIENCE / Life Sciences / General