Human Genome Methods: 1st Edition (Paperback) book cover

Human Genome Methods

1st Edition

By Kenneth W. Adolph

CRC Press

320 pages

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Paperback: 9780849344114
pub: 1997-10-28
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Human Genome Methods is a practical guide to the application of molecular biology and genetics techniques to research on human cells. Written by recognized authorities who often originated the techniques described, chapters present experimental protocols that are readily used at the laboratory bench. The step-by-step protocols are concise and easy to follow to be reproducible by researchers of various levels of expertise. Suggestions for successful application of procedures are included, along with recommended materials and suppliers. Helpful background information and results of applying the methods described are also given.

Section I covers topics such as microsatellite DNA, dynamic mutations, gene targeting using the DNA triple helix, and protease footprinting of DNA-protein interactions. This is followed in Section II by discussions of in situ hybridization, cell synchronization, and cell cycle specific gene expression. Methods concerned with programmed cell death are explored in Section III, which covers this emerging research area and the culture and analysis of cancer cells. Section IV presents methods related to transgene analysis of mouse embryonic stem cells, generation and knockout studies with null mutant mice, and mouse models for human disease. The final section reviews genome mapping, with an emphasis on the construction of linkage maps and on somatic cell hybrids for mapping disease genes.

Table of Contents

DNA Analysis

Human Microsatellite Repeat Markers and Their Application to Analysis of Clonality and Allelic Loss in Tumors, E.P. Henske and D.J. Kwiatkowski

Molecular Detection of Dynamic Mutations, R.F. Kooy, B.A. Oostra, and P.J. Willems

Gene Targeting via Triple Helix Formation, G. Wang and P.M. Glazer

Protease Footprinting Analysis of Protein-Protein and Protein-DNA Interactions, R. Hori and M. Carey

Gene Expression

Studying Gene Expression on Tissue Sections Using In Situ Hybridization, U. Albrecht, H.-C. Lu, J.-P. Revelli, X.-C. Xu, R. Lotan, and G. Eichele

Synchronization of Cells by Elutriation: Analysis of Cell Cycle Specific Gene Expression, S.F. Dowdy, L.f. VanDyk, and G.H. Schreiber

Programmed Cell Death; Cell Culture

Molecular Analysis of Programmed Cell Death in Mammals, R. Gururajan, J.M. Lahti, and V.J. Kidd

An In Vitro Model for Human Bladder Cancer Pathogenesis Studies, T.R. Yeager, D.F. Jarrard, and C.A. Reznikoff

Transgenes, Knockout Mice, and Mouse Models

Transgene Analysis in Mouse Embryonic Stem Cells Differentiating In Vitro, N. Faust, S. Konig, C. Bonifer, and A.E. Sippel

Defining the Function of Related Genes Using Null Mutant Mice, V. Kaartinen, L. Haataja, N. Heisterkamp, and J. Groffen

Genetic Analysis of Mouse Models for Rheumatoid Arthritis, R. Holmdahl et al.

Genome Mapping

Human Genome Mapping, A. Collins and N.E. Morton

Somatic Cell Hybrid Mapping Panels: Resources for Mapping Disease Genes, J. Overhauser

Gene Mapping Using Somatic Cell Hybrids, N.K. Spurr, M. Bouzyk, and D.P. Kelsell


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Subject Categories

BISAC Subject Codes/Headings:
SCIENCE / Life Sciences / Genetics & Genomics
SCIENCE / Research & Methodology
SCIENCE / Life Sciences / Biology / Molecular Biology
SCIENCE / Life Sciences / General