Immune and Receptor Assays in Theory and Practice: 1st Edition (Hardback) book cover

Immune and Receptor Assays in Theory and Practice

1st Edition

By Patrick Englebienne

CRC Press

400 pages

Purchasing Options:$ = USD
Hardback: 9780849300615
pub: 1999-12-28
SAVE ~$51.00
$255.00
$204.00
x

FREE Standard Shipping!

Description

In the past ten years, employment of immuno- and receptor- assays has grown dramatically. Now used in the pharmaceutical industry for automated screening programs, in the agro-food industry for on-line processing control and food adulteration detection, and in clinical laboratories, they are fully integrated analytical tools. However, the literature often covers only one type of assay or just one of the many systems available. Immune and Receptor Assays in Theory and Practice gathers and organizes the available information to help you establish the best assay for your application.

This composite presents the fundamentals of both techniques and introduces practical examples of equation use, antibody and receptor purification, antigen labeling, immunization, and establishment of antibodies for long-term storage. It contrasts the many different assay designs and addresses market trends as the context for developing immuno-assay goals. In addition, this volume summarizes the biochemical and physical properties involved in antibody- and receptor-ligand interactions and reagent manufacture.

This is the first, single-volume synthesis of both immuno- and receptor-assays. With theoretical background and practical examples, Immune and Receptor Assays in Theory and Practice allows you to base your experiment on proven techniques, components, and applications for the most reliable results.

Reviews

"Englebienne has crafted a very easy-reading, user-friendly, and informative text, which I think could be used in teaching a methods course in this area… This will be a welcome introduction to readers new to the field… In summary, this text provides a nice background and reference for individuals who are developing immunoassays and receptor assays for the measurement of analytes."

-Clinical Chemistry, 47, no. 12, 2001

Promo Copy

Table of Contents

INTRODUCTION

RECEPTORS AT WORK

RECEPTORS AND ANTIBODIES

Origin and Relatedness

Structure, Biosynthesis, and Interactions

Biological, Biosynthetic, and Synthetic Alternatives

RECEPTOR AND ANTIBODY PRODUCTION

Cell Culture and Receptor Production

Antigens, Haptens, and Immunogenicity

Production of Monoclonal and Polyclonal Antibodies

Extraction, Purification, and Stabilization of Receptors and Antibodies

MOLECULAR BASIS OF LIGAND-RECEPTOR INTERACTION

The Molecular Recognition Process

Kinetics of Ligand-Receptor Interactions

Physical aspects of Ligand-Receptor Interactions

Factors Influencing Ligand-Receptor Interactions

IMMUNO- AND RECEPTOR-ASSAY DESIGN

Competitive Versus Noncompetitive Assay

Homogeneous Versus Heterogeneous - Liquid Versus Solid Phase Assay

Agglutination and Precipitation Assays

Assays Using Labeled Reagents

Market or Technology Driven Assay Design

ANALYTICAL SIGNAL: GENERATION AND DETECTION

Labels and Their Measurement

Techniques of Labeling

Tracer Purification and Stabilization

Biosensors

Instrumentation

THE SAMPLE

Insoluble Versus Soluble Analyte

Matrix Effects

Standardization

ASSAY VALIDATION

The Reference Method

Specificity and Sensitivity

Interferences

DATA ANALYSIS, PROCESSING, AND, PRESENTATION

The Dose-Response Curve

Binding Data

Reagent Stability Follow-Up

Assay Validation

Subject Categories

BISAC Subject Codes/Headings:
MED008000
MEDICAL / Biochemistry
MED044000
MEDICAL / Immunology
MED071000
MEDICAL / Pharmacology