1st Edition

Immune and Receptor Assays in Theory and Practice



ISBN 9780849300615
Published December 28, 1999 by CRC Press
400 Pages

USD $250.00

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Book Description

In the past ten years, employment of immuno- and receptor- assays has grown dramatically. Now used in the pharmaceutical industry for automated screening programs, in the agro-food industry for on-line processing control and food adulteration detection, and in clinical laboratories, they are fully integrated analytical tools. However, the literature often covers only one type of assay or just one of the many systems available. Immune and Receptor Assays in Theory and Practice gathers and organizes the available information to help you establish the best assay for your application.
This composite presents the fundamentals of both techniques and introduces practical examples of equation use, antibody and receptor purification, antigen labeling, immunization, and establishment of antibodies for long-term storage. It contrasts the many different assay designs and addresses market trends as the context for developing immuno-assay goals. In addition, this volume summarizes the biochemical and physical properties involved in antibody- and receptor-ligand interactions and reagent manufacture.
This is the first, single-volume synthesis of both immuno- and receptor-assays. With theoretical background and practical examples, Immune and Receptor Assays in Theory and Practice allows you to base your experiment on proven techniques, components, and applications for the most reliable results.

Table of Contents

INTRODUCTION
RECEPTORS AT WORK
RECEPTORS AND ANTIBODIES
Origin and Relatedness
Structure, Biosynthesis, and Interactions
Biological, Biosynthetic, and Synthetic Alternatives
RECEPTOR AND ANTIBODY PRODUCTION
Cell Culture and Receptor Production
Antigens, Haptens, and Immunogenicity
Production of Monoclonal and Polyclonal Antibodies
Extraction, Purification, and Stabilization of Receptors and Antibodies
MOLECULAR BASIS OF LIGAND-RECEPTOR INTERACTION
The Molecular Recognition Process
Kinetics of Ligand-Receptor Interactions
Physical aspects of Ligand-Receptor Interactions
Factors Influencing Ligand-Receptor Interactions
IMMUNO- AND RECEPTOR-ASSAY DESIGN
Competitive Versus Noncompetitive Assay
Homogeneous Versus Heterogeneous - Liquid Versus Solid Phase Assay
Agglutination and Precipitation Assays
Assays Using Labeled Reagents
Market or Technology Driven Assay Design
ANALYTICAL SIGNAL: GENERATION AND DETECTION
Labels and Their Measurement
Techniques of Labeling
Tracer Purification and Stabilization
Biosensors
Instrumentation
THE SAMPLE
Insoluble Versus Soluble Analyte
Matrix Effects
Standardization
ASSAY VALIDATION
The Reference Method
Specificity and Sensitivity
Interferences
DATA ANALYSIS, PROCESSING, AND, PRESENTATION
The Dose-Response Curve
Binding Data
Reagent Stability Follow-Up
Assay Validation

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Reviews

"Englebienne has crafted a very easy-reading, user-friendly, and informative text, which I think could be used in teaching a methods course in this area... This will be a welcome introduction to readers new to the field... In summary, this text provides a nice background and reference for individuals who are developing immunoassays and receptor assays for the measurement of analytes."
-Clinical Chemistry, 47, no. 12, 2001

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