Integrated Virus Detection: 1st Edition (Hardback) book cover

Integrated Virus Detection

1st Edition

By Charles H. Wick

CRC Press

255 pages | 184 B/W Illus.

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Hardback: 9781482230055
pub: 2014-11-13
eBook (VitalSource) : 9780429161407
pub: 2014-11-13
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Multiple viruses can be detected concurrently using the Integrated Virus Detection System (IVDS). Integrated Virus Detection describes this technology and provides many examples of applications including a chapter on viruses found in honeybees with descriptions of seasonal and yearly variation. This straightforward technology can be used to detect known, unknown, and unsequenced viruses collected from environmental and other complex biological sources.

This book summarizes more than ten US patents issued for the invention of the IVDS, which is the common name of the electrospray–differential mobility analyzer method. The IVDS is powering mankind’s ability to rapidly detect, measure, and monitor viruses as well as virus-like particles. Three facts make rapid detection possible: virus size, which ranges from 20 to 800 nm.; disparity in each virus species’ particle size thus allowing size data to be used for detection and preliminary identification; and the fact that virus particle density is distinct from other nanoparticles.

The IVDS can ascertain the absence of virion particles, thus presenting compelling evidence of a true negative, which is useful in verifying decontamination and other processes. In addition, large numbers of samples may be processed in an automated fashion, providing an excellent means to prescreen them for judicious targeting of subsequent tests such as PCR or the discriminating method for identifying microbes, which is mass spectrometry proteomics.*

The book is helpful to anyone interested in virus detection, especially in situations where many viral types may coexist.

*Identifying Microbes by Mass Spectrometry Proteomics (CRC Press 2013)


"While this volume superficially resembles a textbook, it is a promotional tool and instructional handbook for the use of a privately-patented device, the integrated virus detection system, which promises to use centrifuging and electrospray-differential mobility analysis to quickly detect viruses and virus-like particles in laboratory settings. The author, whose connection to the patents is unclear, is a retired physical scientist who specialized in biological and chemical warfare for the U.S. Army and the Department of Defense. The vast majority of the text is highly technical. It documents various aspects of the device and the tests run on its ability to detect viruses of various types and other particles. Two final chapters look at experiments with viruses in honeybees, and pulsed-lamp decontamination as measured by the device. There are many black and white charts and tables throughout the book."

Ringgold, Inc. Book News, February 2015

Table of Contents

Detection of Whole Viruses


Historical Background

It Works! The Eureka Moment

Flowchart for Virus Detection

Concept of IVDS Operation

IVDS Systems and Operations

Organization of Book



Descriptions of IVDS Components


Commercial IVDS


Capillary Diameter Effect and IVDS Stability

Electrospray Stability

Proof Concept Using MS2


Physical Basis of Invention

MS2 as Test Virus

Characterization of MS2 Using IVDS

Removing Complex Growth Media

Filtration Characteristics of MS2 Bacteriophage


Concentration of Viruses


Concentrator Device

Cause and Effect Curves


Effects of Temperature on MS2 Survival

Dose Response Curves for MS2 (High pH)

Dose Response Curves for MS2 (Low pH)

Isolating Single Viruses from Multivirus Mixtures



Differential Mobility Separation



Analyzing Various Viruses



Sample Analysis Procedures

Analyses of and Results for Specific Viruses

Analysis of Complex Media

Water Sample Analysis

Recovery of Viruses from Surfaces

Detection of Viruses in Bark Beetles

Detection of Other Nanoparticles


Particle Size Results: Comparison of Malvern Nano ZS

Direct Counting of Pili


Detecting Pili

Testing Procedures


Detecting Viruses in Honeybees

Introduction and Formation of BVS, Inc.

Detection of Viruses from Single Honeybee

Rosetta Stone Project

Honeybee Nutrition Study

Essential Oils Study

Snapshots of Viral Loads in Honeybees


Pulsed Lamp Decontamination




Discussion and Conclusions

Current and Future Trends


Potential Applications of IVDS

New RapidDx Instrument

The Future

About the Author


Charles H. Wick is a retired lieutenant colonel and senior scientist from the U.S. Army Edgewood Chemical Biological Center. He earned his PhD from the University of Washington. Dr. Wick is best known for work in forensic science done in concert with the Department of Defense. Dr. Wick’s research work has resulted in international recognition as an authority on individual performance for operations conducted on a nuclear, biological, and chemical battlefield. He has made lasting and important contributions to forensic science and antiterrorism and holds several U.S. patents in the area of microbe detection and classification. He has written more than 45 civilian and militarypublications and has received various awards and citations.

Subject Categories

BISAC Subject Codes/Headings:
MEDICAL / Infectious Diseases
SCIENCE / Research & Methodology
SCIENCE / Life Sciences / Biology / Microbiology