Lysosomes are key subcellular organelles that regulate the cell function. Many of the essential activities of the cell are dependent on lysosomes. Dysfunction is linked to multiple diseases - storage disorders, neurodegeneration, immunological diseases and cancer. This book discusses concepts and methods used to study lysosome ion and small molecule transport. The contents will not only attract accomplished investigators in need of a broad review and synthesis of this important subject but will also appeal to young investigators and trainees needing to acquire comprehensive knowledge and technical skills working with lysosomal ion channels and small molecule transporters.
Key selling features:
Electrophysiological recording of endolysosomal channels. Endolysosmal two-pore channels and their regulation by mechanistic target of rapamycin (mTOR). Chloride transport across the lysosomal membrane. Mucolipin channels in lysosome storage disease. Ion channels of melanosomes and melanin formation. NAADP-mediated lysosomal Ca2+ release and NAADP receptors. Endolysosomal channels critical for endolysotosis and recycling. Mechanism of Ebola virus invasion via endocytic pathway and lysosome escape. The lysosomal H+ ATPase. Amino acid transport across the lysosomal membrane. Amino acid sensing at the lysosomal membrane. Cholesterol transport across the lysosomal membrane. Lysosomal peptide translocation. Chaperonemediated autophagy. Lysosomal membrane permeabilization.