1st Edition
Ion and Molecule Transport in Lysosomes
Lysosomes are key subcellular organelles that regulate the cell function. Many of the essential activities of the cell are dependent on lysosomes. Dysfunction is linked to multiple diseases - storage disorders, neurodegeneration, immunological diseases and cancer. This book discusses concepts and methods used to study lysosome ion and small molecule transport. The contents will not only attract accomplished investigators in need of a broad review and synthesis of this important subject but will also appeal to young investigators and trainees needing to acquire comprehensive knowledge and technical skills working with lysosomal ion channels and small molecule transporters.
Key selling features:
Editors
Contributors
1. Endosomal and Lysosomal Electrophysiology
Xiaoli Zhang, Mingxue Gu, Meiqin Hu, Yexin Yang, and Haoxing Xu
2. Chloride Transport across the Lysosomal Membrane
Sonali Saha, Anja Blessing, and Thomas J. Jentsch
3. Endolysosomal Patch Clamping: Approaches to Measure Vesicular Ion Channel Activities
Cheng-Chang Chen, Christian Grimm, Christian Wahl-Schott and Martin Biel
4. TRPML Subfamily of Endolysosomal Channels: Concepts and Methods
Nicholas E. Karagas, Morgan A. Rousseau, and Kartik Venkatachalam
5. Investigating the Role of Two-Pore Channel 2 (TPC2) in Zebrafish Neuromuscular Development
Sarah E. Webb, Jeffrey J. Kelu, and Andrew L. Miller
6. Functional Study of Lysosomal Nutrient Transporters
Xavier Leray, Corinne Sagné, and Bruno Gasnier
7. Lysosomal Vitamin B12 Trafficking
Sean Froese and Matthias R. Baumgartner
8. Detection of Lysosomal Membrane Permeabilization
Anne-Marie Ellegaard, Line Groth-Pedersen, and Marja Jäättelä
9. Rapid Isolation of Lysosomes from Cultured Cells Using a Twin Strep Tag
Jian Xiong, Jingquan He, Michael X. Zhu, and Guangwei Du
10. A Transcriptomic Analysis and shRNA Screen for Intracellular Ion Channels and Transporters Regulating Pigmentation
Donald C. Koroma, Salwa Y. Hafez, and Elena Oancea
Index
Biography
Bruno Gasnier is CNRS Research Director, Group Leader at CNRS/Paris Descartes.
Michael Xi Zhu is currently with the Department of Integrative Biology and Pharmacology at the University of Texas Health Science Center at Houston.