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Laboratory Methods for the Detection of Mutations and Polymorphisms in DNA: 1st Edition (Hardback) book cover

Laboratory Methods for the Detection of Mutations and Polymorphisms in DNA

1st Edition

By Graham R. Taylor

CRC Press

352 pages

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Hardback: 9780849392337
pub: 1997-01-24
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The analysis of DNA sequence polymorphisms and mutations is of central importance in understanding biological systems. This book is devoted to the experimental analysis of DNA and presents easy-to-follow protocols. Various techniques from the simple to the highly complex are detailed in this volume, providing a wide spectrum of available methods and practical advice. The methods are described in terms of:

  • History and background

  • Principles and theory

  • Equipment and reagents

  • Protocols

  • Troubleshooting

  • Applications

  • Improvements

  • Results

  • Comparisons with other methods

  • Future prospects and developments

    This is an essential manual for researchers working in human, animal, or plant molecular genetics and is particularly valuable for hospital and commercial laboratories.

  • Table of Contents

    Introduction, G.R. Taylor

    Arbitrarily Primed PCR Fingerprints, F. Mathieu-Daude, D. Ralph, and M. McClelland

    Minisatellite Analysis, G. Rysiecki, W.P. Childs, and N. Duxbury

    Microsatellite Analysis, S. Payne

    ARMS Analysis of Point Mutations, S. Little

    Solid Phase Minisequencing, A.C. Syvanen

    Oligonucleotide Ligation Assay, E.S. Winn-Deen

    Combined Single-Strand Conformation Polymorphism and Heteroduplex Analysis, A.J. Wallace

    Denaturing Gradient Gel Electrophoresis (DGGE), M. Loosekoot and R. Fodde

    Coupled Transcription-Translation, T. Hamzehleoi and S. West

    Peptide Nucleic Acids, H. Orum, T Koch, M. Egholm, H. O'Keefe, and J. Coull

    Orphan Peak Analysis, M. Hattori, H. Hagiwara, and Y. Sakaki

    Sequence-Specific Gene Signatures Produced by LSSP-PCR, A.J.G. Simpson and S.D.J. Pena

    Bisulphite Genomic Sequencing of Methylated Cytosines, S.J. Clark and M. Frommer

    Direct Sequencing of PCR Products, E. Werle

    Heterozygote Determination Using Automated DNA Sequencing Technology, M.N. Kronick

    A Robust Sequencing Protocol for PCR Products, M.D. Robinson and K.J. Leach

    Use of MutY and Thymine Glycosylase to Detect Point Mutations, I.C. Hsu

    MutS-Exonuclease Protection for the Detection of Mismatched Heteroduplexes, V.H. Laitinen, G.R. Taylor, S. Hamilton and P. Flick

    Enzyme Mismatch Cleavage-Bacterial Resolvases, R.D. Mashal and J. Sklar

    Chemical Cleavage of Mismatch in Heteroduplexes, J. Prosser

    Comparison of Mutation Detection by Chemical Cleavage of Mismatch (CCM) and Enzyme Mismatch Cleavage (EMC), R. Youil, S.J. Ramus, and R.G.H. Cotton

    Analysis of DNA Sequence Using Biosensors, P. Nilsson, B. Persson, M. Uhlen, and P.A. Nygren

    Repeat Expansion Detection, C. Zander, C. Erickson Burgess, J. Johansen, K. Lindblad, T. Hudson, and M. Schalling

    Comparative Genomic Hybridization, A Kallioniemi, R. Karhu, and O.-P. Kallioniemi

    Large-Scale Electrophoretic Studies: Microplate Array Diagonal Gel Electrophoresis (MADGE) and Profiling of Oligonucleotide Dissociation by Gel Electrophoresis (PODGE), I.N.M. Day, S.D. O'Dell, G.P. Weavind, and S.E. Humphries

    DNA Sequence Database Searches, Comparisons, and Primer Design, G. Williams

    Accessing Genome Data: The Human Genome Database, Internet, and WWW, G. Williams

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    Subject Categories

    BISAC Subject Codes/Headings:
    MEDICAL / Biochemistry
    SCIENCE / Life Sciences / Genetics & Genomics
    SCIENCE / Research & Methodology
    SCIENCE / Life Sciences / General