Laboratory Methods for the Detection of Mutations and Polymorphisms in DNA  book cover
1st Edition

Laboratory Methods for the Detection of Mutations and Polymorphisms in DNA

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ISBN 9780849392337
Published January 24, 1997 by CRC Press
352 Pages

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Book Description

The analysis of DNA sequence polymorphisms and mutations is of central importance in understanding biological systems. This book is devoted to the experimental analysis of DNA and presents easy-to-follow protocols. Various techniques from the simple to the highly complex are detailed in this volume, providing a wide spectrum of available methods and practical advice. The methods are described in terms of:

  • History and background
  • Principles and theory
  • Equipment and reagents
  • Protocols
  • Troubleshooting
  • Applications
  • Improvements
  • Results
  • Comparisons with other methods
  • Future prospects and developments
    This is an essential manual for researchers working in human, animal, or plant molecular genetics and is particularly valuable for hospital and commercial laboratories.
  • Table of Contents

    Introduction, G.R. Taylor
    Arbitrarily Primed PCR Fingerprints, F. Mathieu-Daude, D. Ralph, and M. McClelland
    Minisatellite Analysis, G. Rysiecki, W.P. Childs, and N. Duxbury
    Microsatellite Analysis, S. Payne
    ARMS Analysis of Point Mutations, S. Little
    Solid Phase Minisequencing, A.C. Syvanen
    Oligonucleotide Ligation Assay, E.S. Winn-Deen
    Combined Single-Strand Conformation Polymorphism and Heteroduplex Analysis, A.J. Wallace
    Denaturing Gradient Gel Electrophoresis (DGGE), M. Loosekoot and R. Fodde
    Coupled Transcription-Translation, T. Hamzehleoi and S. West
    Peptide Nucleic Acids, H. Orum, T Koch, M. Egholm, H. O'Keefe, and J. Coull
    Orphan Peak Analysis, M. Hattori, H. Hagiwara, and Y. Sakaki
    Sequence-Specific Gene Signatures Produced by LSSP-PCR, A.J.G. Simpson and S.D.J. Pena
    Bisulphite Genomic Sequencing of Methylated Cytosines, S.J. Clark and M. Frommer
    Direct Sequencing of PCR Products, E. Werle
    Heterozygote Determination Using Automated DNA Sequencing Technology, M.N. Kronick
    A Robust Sequencing Protocol for PCR Products, M.D. Robinson and K.J. Leach
    Use of MutY and Thymine Glycosylase to Detect Point Mutations, I.C. Hsu
    MutS-Exonuclease Protection for the Detection of Mismatched Heteroduplexes, V.H. Laitinen, G.R. Taylor, S. Hamilton and P. Flick
    Enzyme Mismatch Cleavage-Bacterial Resolvases, R.D. Mashal and J. Sklar
    Chemical Cleavage of Mismatch in Heteroduplexes, J. Prosser
    Comparison of Mutation Detection by Chemical Cleavage of Mismatch (CCM) and Enzyme Mismatch Cleavage (EMC), R. Youil, S.J. Ramus, and R.G.H. Cotton
    Analysis of DNA Sequence Using Biosensors, P. Nilsson, B. Persson, M. Uhlen, and P.A. Nygren
    Repeat Expansion Detection, C. Zander, C. Erickson Burgess, J. Johansen, K. Lindblad, T. Hudson, and M. Schalling
    Comparative Genomic Hybridization, A Kallioniemi, R. Karhu, and O.-P. Kallioniemi
    Large-Scale Electrophoretic Studies: Microplate Array Diagonal Gel Electrophoresis (MADGE) and Profiling of Oligonucleotide Dissociation by Gel Electrophoresis (PODGE), I.N.M. Day, S.D. O'Dell, G.P. Weavind, and S.E. Humphries
    DNA Sequence Database Searches, Comparisons, and Primer Design, G. Williams
    Accessing Genome Data: The Human Genome Database, Internet, and WWW, G. Williams
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    Taylor\, Graham R.