Laboratory Techniques in Plant Bacteriology
Laboratory Techniques in Plant Bacteriology is ideal for scientists and students who seek a career in plant pathogenic bacteria. This book contains 41 chapters comprising practicable techniques from isolation of bacterial plant pathogens to their identification up to species and race/biotype level. It includes identification protocols of morphological, biochemical, immunological, and molecular-based techniques.
This book comprises all technological aspects of plant bacteriological studies. Its content is ideal for graduate students and research scholars including bacteriological professionals or technicians. The book ultimately provides working technologies useful for controlling bacterial disease pathogens.
Laboratory Ethics in Plant Bacteriology Laboratory, Instruments, and General Guidelines. Confirmation of Bacterial Pathogen in Diseased Sample. Histopathology of Bacterial Infection. Sterilization. Medium for Isolation of Plant Pathogenic Bacteria. Isolation of Bacterial Plant Pathogens. Purification of Plant Pathogenic Bacterial Cultures. Rapid Assessment of Plant Pathogenic Nature of Bacterial Isolates. Determination of Pathogenesity of the Isolated Bacterial Culture on Natural Host Plant. Isolation and Enumeration of Microorganisms Associated with Bacterial Plant Pathogens from Soil, Rhizosphere, and Phylloplane. Preparation of Pure Cultures of Microorganisms. Maintenance and Preservation of Bacterial Pure Cultures. Staining of Bacterial Cultures for Morphological Studies. Bacterial Mobility. Describing Bacterial Colony Morphology. Physiological Characterization of Bacteria. Biochemical Test Used in Identification of Bacteria. Characterization of Phytopathogenic Bacteria up to Genus. Differentiation of Bacterial Genus up to Group. Differentiation of Bacterial Genus up to Species. Identification of Races of Bacterial Plant Pathogen. Studies on Bacterial Cell Wall Related Biological Compounds. Isolation of Bacterial Metabolite. Role of Bacterial Component in Induction of Disease Reaction or Pathogenesis. Enumeration of Bacterial Population. Determination of Ice-Nucleation in Plant Pathogenic Bacteria. Transmission of Plant Pathogenic Bacteria through Vector. Determination of Perpetuation of Bacterial Plant Pathogen. Determination of Plant Resistance. Identification of Bacteria by Using Immunodiagnostic Technique. Identification of Bacteria by Using Molecular Techniques. Bacteriophages of Plant Pathogenic Bacteria. Determination of Bacterial Sensitivity to Antibiotics and Pesticide, Determination of Mutation in Bacterial Plant Pathogen. Transfer of Genetic Factor in Plant Pathogenic Bacteria. Population Studies of Plant Pathogenic Bacteria on Host Plant. Preservation of Bacterial Disease Samples. Measurement of Plant Bacterial Disease. Isolation of Antibacterial Compounds. Isolation of Antibacterial Microbes. Preparation of Bactericidal Formulations.