452 Pages 58 B/W Illustrations
    by CRC Press

    452 Pages 58 B/W Illustrations
    by CRC Press

    As the highly anticipated update to Lipid Second Messengers (CRC Press, 1999), Lipid-Mediating Signaling is a current and comprehensive overview of research methods used in lipid-mediated signal transduction. Pioneering experts provide a much-needed distillation of a decade’s worth of advances in research techniques that are pertinent in understanding how lipid-mediated signal transduction ties to pathologic disorders.

    Part I describes methods used to identify activities of and assay the expression of different enzymes involved in lipid-mediated signaling. Part II highlights the use of mass spectrometry to ascertain the lipid content in various systems. Part III contains two chapters devoted to techniques used to determine the role of lipids in the activation of gene transcription. Part IV rounds out the book’s solid coverage by focusing on methods used to assess fatty acid uptake and metabolism.

    Lipid-Mediating Signaling is an unrivaled reference for this rapidly expanding, yet resource-limited field, and it is a valuable resource for for cell biologists, biochemists, and pharmacologists, as well as for researchers studying signaling transduction.

    ENZYME SYSTEMS INVOLVED IN LIPID-MEDIATED SIGNALING
    Methods for Measuring the Activity and Expression of Phospholipases A2

    Prerna Rastogi, Janhavi Sharma, and Jane McHowat
    Methods of Measuring the Activity and Expression of Phospholipases C
    Ryan A. Frieler, John A. Watt, and Thad A. Rosenberger
    Methods for Measuring the Activity and Expression of Phospholipase D
    Wenjuan Su and Michael A. Frohman
    Methods for Measuring the Activity and Expression of Diacylglycerol Kinase
    Kaoru Goto, Yasukazu Hozumi, Tomoyuki Nakano, Alberto M. Martelli, and Hisatake Kondo
    Methods for Measuring Endocannabinoid Production and Expression and Activity of Enzymes Involved in the Endocannabinoid System
    Tiziana Bisogno, Luciano De Petrocellis, and Vincenzo Di Marzo
    MASS SPECTROMETRY METHODS FOR LIPID ANALYSIS
    Mass Spectrometry Methods for the Analysis of Phospholipid Molecular Species Analysis: A Shotgun Lipidomics Approach
    Xianlin Han, Hua Cheng, Xuntian Jiang, and Youchun Zeng
    Mass Spectrometry Methods for the Analysis of Bioactive Sphingolipids: A High-Performance Liquid Chromatography/Tandem Mass Spectrometry Approach
    Jacek Bielawski, Motohiro Tani, and Yusuf A. Hannun
    Mass Spectrometry Methods for the Analysis of Oxidant Stress in Biological Fluids and Tissues: Quantification of F2-Isoprostanes and F4-Neuroprostanes Using Mass Spectrometry
    Ginger L. Milne and Jason D. Morrow
    LIPID-MEDIATED REGULATION OF GENE EXPRESSION
    Methods for Assessing the Role of Lipid-Activated Nuclear Receptors in the Regulation of Gene Transcription
    Jenny Kaeding, Jocelyn Trottier, and Olivier Barbier
    Fluorescence Methods to Assess the Impact of Lipid Binding Proteins on Ligand-Mediated Activation of Gene Expression
    Heather A. Hostetler, Avery L. McIntosh, Anca D. Petrescu, Huan Huang, Barbara P. Atshaves, Eric J. Murphy, Ann B. Kier, and Friedhelm Schroeder
    METHODS TO ASSESS FATTY ACID AND PHOSPHOLIPID METABOLISM
    Methods for Measuring Fatty Acids Using Chromatographic Techniques
    Gwendolyn Barceló-Coblijn, Cameron C. Murphy, and Eric J. Murphy
    Methods for Measuring Fatty Acid Uptake and Targeting in Cultured Cells and In Vivo
    Paul G. Millner, Serena M. Lackman, and Eric J. Murphy
    Methods for Kinetic Analysis of Fatty Acid Incorporation and Turnover In Vivo: A Steady-State Kinetic Radiotracer Approach
    Dhaval P. Bhatt and Thad A. Rosenberger

    Biography

    Eric J. Murphy, University of North Dakota, Grand Forks, USA
    Thad A. Rosenberger, University of North Dakota, Grand Forks, USA