Although the role of liposomes in drug targeting has been discussed extensively in several reviews and books, there has been no comprehensive coverage of related methodology. This book constitutes the first attempt to put together all aspects of lipsome technology as applied to medical sciences. Volume III is devoted to the growing variety of techniques yielding targeted liposomes and to approaches of studying liposomal behaviour in the biological milieu both in vitro and in vivo.
1. The Challenge of Liposome Targeting In Vivo 2. Covalent Coupling of Monoclonal Antibodies and Protein A to Liposomes: Specific Interaction with Cells In Vitro and In Vivo 3. Chemical Methods for the Modification of Liposomes with Proteins or Antibodies 4. Coupling of Antibodies with Liposomes 5. Preparation and Analysis of Antibody-Targeted Liposomes 6. Immobilization of Specific Proteins on Liposome Surface: Systems for Drug Targeting 7. Modification of Liposome Surface Properties by Synthetic Glycopilids 8. Preparation of Glycoside-Bearing Liposomes for Targeting 9. Methodology Involved in Biotin-Conjugated Phospholipids, Glycolipids, and Gangliosides 10. The Design and Characterization of Temperature Sensitive Liposomes 11. Theoretical and Practical Considerations in Preparing Liposomes for the Purpose of Releasing Drug in Response to Changes in Temperature and pH 12. Calcein as a Tool in Liposome Methodology 13. Self-Quenching of Carboxyfluorescein Fluorescence: Uses in Studying Liposome Stability and Liposome Cell Interaction 14. Interactions of Lipsomes with Plasma Proteins 15. Methodological Aspects Dealing with Stability Measurements of Liposomes In Vitro Using the Carboxyfluorescein-Assay 16. The Use of Gamma Ray Perturbed Angular Correlation Technique for the Sudy of Liposomal Integrity In Vitro and In Vivo 17. Methodology in Assessing Liposomal Stability in the Presence of Blood, Clearance from the Circulation of Injected Animals, and Uptake by Tissues