Manual of Immunological Methods: 1st Edition (Paperback) book cover

Manual of Immunological Methods

1st Edition

By Canadian Networking

CRC Press

160 pages

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Paperback: 9780849385582
pub: 1998-07-02
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Description

The Manual of Immunological Methods represents the collaboration of the Canadian Network of Toxicology Centers, a non-profit network of university-based scientists dedicated to research, training, risk assessment, and communication. This manual provides detailed immunological methods that can be utilized by researchers or practitioners who want to enhance the successful application of this science. It emphasizes the need for continuously improving the quality of experiments performed and maintaining consistency in the results obtained.

The Manual of Immunological Methods presents validated assays that are accompanied by a series of tools that will guide readers in performing their own laboratory work in the spirit of the Good Laboratory Practices. It covers specific methodologies including sample collection, preparation of cells, assay conditions, and data evaluation. Discussion of species differences and comparisons of a wide variety of species is an important feature. Both graduate students and practicing researchers in the areas of immunology and immunotoxicology will appreciate the Manual of Immunological Methods as a guide to standard operating procedures in the laboratory.

Table of Contents

Identification, Anaesthesia, and Euthanasia

Identification of Laboratory Rodents by Ear Notching

Identification of Fish by Fin Clipping

Anaesthesia of Fish and Rodents

Euthanasia of Rodents

Euthanasia of Fish

Working Sheet: Weight of Laboratory Animals

Collection of Peripheral Blood Samples

Reagents

Materials and Equipment

Procedure

Removal of Organs

Reagents

Materials and Equipment

Procedure

Preparation of Cell Suspensions

Extrusion of Coelomocytes in the Earthworm

Hemocyte Collection in the Mollusk

Cell Suspension from Peritoneal Exudate

Isolation of White Blood Cells from Peripheral Blood

Cell Suspensions from Lymphoid Organs

Assessment of Cell Viability

Determination of Cell Viability and Concentration by Trypan Blue Dye Exclusion

Determination of Cell Viability and Cell Concentration with Ethidium Bromide and Acridine Orange

Flow Cytometric Assessment of Cell Viability

Working Sheet: Cell Viability and Concentration by Trypan Blue Dye Exclusion or Ethidium Bromide/Acridine Orange

Cell Cryopreservation

Reagents

Materials and Equipment

Procedure

Special Recommendations

Analysis of Results

Species-Related Changes in the Protocol

Technique-Related Changes in the Protocol: Phagocytosis with Yeast

Statistical Tests

Working Sheet: Phagocytic Function

Oxidative Burst Assay Using Flow Cytometry

Reagents

Materials and Equipment

Procedure

Special Recommendations

Analysis of Results

Species-Related Changes in the Protocol

Statistical Tests

Working Sheet: Acquisition List

Cell Cytotoxicity

Natural Killer (NK) Cell Activity

Antibody-Dependent Cellular Cytotoxicity

Lymphokine-Activated Killer (LAK)

Working Sheet: Acquistion List

Lymphoblastic Transformation

Reagents

Materials and Equipment

Procedure

Analysis of Result

Special Recommendations

Species-Related Changes in the Protocol

Working Sheet: Mitogenic Assay

Determination of Antibody-Producing Cells to a Specific Antigen

Liquid Plaque Forming Cell Assay in Mouse Model

Agar Plaque Forming Cell Assay in Rat Model

Working Sheet: Plaque Forming Cells (PFC)

Mixed Lymphocyte Reaction (MLR)

Reagents

Materials and Equipment

Procedure

Analysis of Results

Special Recommendations

Species-Related Changes in the Protocol

Working Sheet: Mixed Lymphocyte Reaction

Intracellular Levels of Calcium Assay Using Flow Cytometry

Reagents

Materials and Equipment

Procedure

Special Recommendations

Analysis of Results

Species-Related Changes in the Protocol

Statistical Tests

Working Sheet: Acquisition List

Phenotyping of Blood Mononuclear Cells

Reagents

Materials and Equipment

Procedure

Special Recommendations

Analysis of Results

Species-Related Changes in the Protocol

Immunophenotype of Rat Peripheral Blood Lymphocytes

Working Sheet

Evaluation of Intracellular Level of Thiols

Reagents

Materials and Equipment

Procedure

Special Recommendations

Analysis of Results

Species-Related Changes in the Protocol

Working Sheet: Acquisition List

Apoptosis

Reagents

Materials and Equipment

Procedure

Analysis of Results

About the Series

Handbooks in Pharmacology and Toxicology

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Subject Categories

BISAC Subject Codes/Headings:
MED000000
MEDICAL / General
MED044000
MEDICAL / Immunology
SCI043000
SCIENCE / Research & Methodology