Mass Spectrometry–Based Glycoproteomics and Its Clinic Application
- Available for pre-order. Item will ship after July 29, 2021
As one of the most extensive and important protein post-translational modifications, glycosylation plays very important roles in the organism and is associated with different physiological and pathological processes. Therefore, there has been increasing attention and need to characterize protein glycosylation sites, structures and their modification degree, in order to understand well their biological functions or look for potential biomarkers for disease diagnosis and treatment. Mass spectrometry (MS) is one of the most powerful tools to study biomolecules including glycoproteins and glycans. With the continuous development of glycoproteomics and glycomics based on MS analysis, more and more techniques have appeared and contribute to understanding the structure and function of glycoproteins and glycans. Therefore, this book demonstrates the progress that has been achieved in MS-based glycoproteomic analysis, including a wide range of analytical methodologies and strategies involved in selective enrichment, qualitative analysis, quantitative analysis and data analysis, together with their clinical applications. And some significant examples will be discussed to illustrate the principles, laboratory protocols and key implementation advice to ensure successful results. This book will serve as a valuable resource to appropriate techniques and their applications for students, postdocs, and researchers working in proteomics, glycoscience, analytical chemistry, biochemistry, and clinical medicine, and so on.
Table of Contents
1. Selective enrichment methods for N-glycosite containing peptides in N-glycoproteomics 2. Quantitative methods for N-glycosite containing peptides in N-glycoproteomics 3. Qualitative and quantitative methods for N-glycans in N-glycomics 4. Qualitative and quantitative analytical methods for intact glycopeptides 5. Analytical Software and Databases in N-glycoproteomics 6. Clinical Applications
Haojie Lu is a professor at Fudan University, and he specializes in proteomics based on mass spectrometry with particular emphasis on novel technologies for separation and identification of low-abundant proteins and post-translationally modified proteins (including glycosylation), as well as relative and absolute quantification methods for proteomics.