1st Edition

Membrane Fusion

By Jan Wilschut Copyright 1990

    This balanced volume provides a broad and coherent overview of recent progress in membrane fusion research—highlighting an interdisciplinary treatment of the subject from the fields of biophysics, biochemistry, cell biology, virology, and biotechnology—in a single volume., Featuring easy-access sections on the general properties of membranes and applications of membrane fusion techniques, this valuable sourcebook outlines membrane structure, lipid polymorphism, and intermembrane forces ... covers membrane fusion in model systems ... presents the fusogenic properties of enveloped viruses ... discusses the fusion and flow of intracellular membranes and cell-cell fusion occurring during fertilization and myogenesis ... offers applications of membrane fusion techniques in cell-biological research and biotechnology ... and more., Supplying a comprehensive view of this exciting topic, Membrane Fusion is a working resource for molecular, cell, and membrane biologists; biophysicists; biochemists; virologists; biotechnologists; microbiologists; immunologists; physiologists; and graduate and medical school students in biophysics, biochemistry, physiology, virology, cell biology, and biotechnology.

    Preface, Contributors, Introduction, I. GENERAL ASPECTS OF MEMBRANE STRUCTURE AND FUNCTION, 1. Lipid and Protein Structure of Biological Membranes, 2. Lipid Polymorphism, 3. Forces Governing Lipid Interaction and Rearrangement, II. MEMBRANE FUSION IN MODEL SYSTEMS, 4. Membrane Fusion in Lipid Vesicle Systems: An Overview, 5. Modeling of Aggregation and Fusion of Phospholipid Vesicles, 6. Role of Nonbilayer Lipids in Membrane Fusion, 7. Fusion of Zwitterionic Phospholipid Vesicles, 8. Fusion of Phospholipid Vesicles with Planar Phospholipid Membranes, 9. Fusion of Liposomes Induced and Modulated by Proteins and Polypeptides, 10. Effects of an Integral Membrane Glycoprotein on Phospholipid Vesicle Fusion, 11. Membrane Fusion Induced by Polyethylene Glycol, 12. Modulation of Membrane Fusion by Glycolipids and Lectin-Mediated Intermembrane Contact, III. FUSOGENIC PROPERTIES OF VIRUSES, 13. Membrane Fusion and the Infectious Entry of Viruses into Cells: An Overview, 14. Fusion of Enveloped Viruses: From Microscopic Observation to Kinetic Simulation, 15. Influenza Virus Hemagglutinin and Membrane Fusion, 16. Fusion of Viruses with Phospholipid Vesicles at Neutral pH, 17. The Role of Envelope Glycoproteins in the Fusion of Sendai Virus with Liposomes, 18. Functional Reconstitution of Viral Envelopes, IV. INTRACELLULAR MEMBRANE TRAFFIC, 19. Membrane Cycling Through the Endocytotic and Exocytotic Pathways: An Overview, 20. Cell-Free Systems for Studying the Pathway of Receptor-Mediated Endocytosis, 21. Specific Conditions for Fusion of Membranes of Nuclear Envelopes, Endoplasmic Reticulum, and Golgi Apparatus from Vertebrate Cells, 22. Reconstitution of Transport Between Early Compartments of the Secretory Pathway Using Cell-Free Systems, 23. Cytoplasmic Determinants of Exocytotic Membrane Fusion, 24. Permeabilized Cells: An Approach to the Study of Exocytosis, 25. Ultrastructural Aspects of Exocytosis, V. CELL-CELL FUSION, 26. Fusion of Myoblasts, 27. Fusion of Sperm and Egg Plasma Membranes During Fertilization, VI. APPLICATIONS OF MEMBRANE FUSION TECHNIQUES, 28. Experimental and Technological Applications of Cell-Cell Fusion: An Overview, 29. Electrofusion and Electropermeabilization in Genetic Engineering, 30. The Production of Monoclonal Antibodies, 31. pH-Sensitive Liposomes: Introduction of Foreign Substances into Cells, 32. Sendai Virus Envelopes as a Biological Carrier: Reconstitution, Targeting, and Application, 33. Enveloped Viruses as a Tool in the Study of Lipid Organization in the Plasma Membrane of Epithelial Cells, 34. Structure and Function of Bacterial Membranes: Insertion of Exogenous Lipids and Proteins by Fusion with Lipid Vesicles, 35. Modulation and Analysis of Structure and Function of the Inner Mitochondrial Membrane Through the Application of Phospholipid Enrichment and Membrane Fusion Techniques, 36. Liposomal Drug Delivery: Current Status and Future Prospects, Index


    JAN WILSCHUT is an Associate Professor in the Department of Physiological Chemistry, University of Groningen, Groningen, The Netherlands. The author or coauthor of over 60 scientific journal articles and book chapters, his research interests include the structure and function of membranes, virus-cell interactions, and liposome-based vaccine development. He received the B.Sc. degree (1970) in chemistry and M.Sc. degree (1973) in biochemistry and immunology from the University of Utrecht, The Netherlands, and Ph.D. degree (1978) in biochemistry from the University of Groningen., DICK HOEKSTRA is an Associate Professor of Physiological Chemistry, University of Groningen, Groningen, The Netherlands. The author of several publications on the development of kinetic fluorescence assays to monitor membrane fusion, fusion mechanisms of artificial and biological membranes, and intracellular membrane flow and (glyco-)lipid trafficking, he is a member of the Scientific Board of the Dutch Society for Multiple Sclerosis Research. He received the M.Sc. degree (1976) in biochemistry and Ph.D. degree (1980) in physiological chemistry from the University of Groningen.